1pjy

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{{Seed}}
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{{STRUCTURE_1pjy| PDB=1pjy | SCENE= }}
{{STRUCTURE_1pjy| PDB=1pjy | SCENE= }}
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'''Solution structure of the HIV-1 frameshift inducing stem-loop RNA'''
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===Solution structure of the HIV-1 frameshift inducing stem-loop RNA===
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==Overview==
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The translation of reverse transcriptase and other essential viral proteins from the HIV-1 Pol mRNA requires a programmed -1 ribosomal frameshift. This frameshift is induced by two highly conserved elements within the HIV-1 mRNA: a slippery sequence comprised of a UUUUUUA heptamer, and a downstream stem-loop structure. We have determined the structure of the HIV-1 frameshift inducing RNA stem-loop, using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The 22 nucleotide RNA solution structure [root mean squared deviation (r.m.s.d.) = 1.2 A] was determined from 475 nuclear Overhauser effect (NOE)-derived distance restrains, 20 residual dipolar couplings and direct detection of hydrogen bonds via scalar couplings. We find that the frameshift inducing stem-loop is an A-form helix capped by a structured ACAA tetraloop. The ACAA tetraloop is stabilized by an equilateral 5' and 3' stacking pattern, a sheared A-A pair and a cross-strand hydrogen bond. Unexpectedly, the ACAA tetraloop structure is nearly identical to a known tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae.
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The line below this paragraph, {{ABSTRACT_PUBMED_12888491}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12888491 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12888491}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA].
==Reference==
==Reference==
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[[Category: Rna structure]]
[[Category: Rna structure]]
[[Category: Tetraloop]]
[[Category: Tetraloop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:10:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:52:21 2008''

Revision as of 01:52, 28 July 2008

Image:1pjy.png

Template:STRUCTURE 1pjy

Solution structure of the HIV-1 frameshift inducing stem-loop RNA

Template:ABSTRACT PUBMED 12888491

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:12888491

Page seeded by OCA on Mon Jul 28 04:52:21 2008

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