1u9p

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[[Image:1u9p.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1u9p| PDB=1u9p | SCENE= }}
{{STRUCTURE_1u9p| PDB=1u9p | SCENE= }}
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'''Permuted single-chain Arc'''
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===Permuted single-chain Arc===
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==Overview==
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We designed a single-chain variant of the Arc repressor homodimer in which the beta strands that contact operator DNA are connected by a hairpin turn and the alpha helices that form the tetrahelical scaffold of the dimer are attached by a short linker. The designed protein represents a noncyclic permutation of secondary structural elements in another single-chain Arc molecule (Arc-L1-Arc), in which the two subunits are fused by a single linker. The permuted protein binds operator DNA with nanomolar affinity, refolds on the sub-millisecond time scale, and is as stable as Arc-L1-Arc. The crystal structure of the permuted protein reveals an essentially wild-type fold, demonstrating that crucial folding information is not encoded in the wild-type order of secondary structure. Noncyclic rearrangement of secondary structure may allow grouping of critical active-site residues in other proteins and could be a useful tool for protein design and minimization.
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(as it appears on PubMed at http://www.pubmed.gov), where 15689399 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15689399}}
==About this Structure==
==About this Structure==
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[[Category: Arc]]
[[Category: Arc]]
[[Category: Parc]]
[[Category: Parc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:56:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:53:09 2008''

Revision as of 01:53, 28 July 2008

Image:1u9p.png

Template:STRUCTURE 1u9p

Permuted single-chain Arc

Template:ABSTRACT PUBMED 15689399

About this Structure

Full crystallographic information is available from OCA.

Reference

Consolidating critical binding determinants by noncyclic rearrangement of protein secondary structure., Tabtiang RK, Cezairliyan BO, Grant RA, Cochrane JC, Sauer RT, Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2305-9. Epub 2005 Feb 2. PMID:15689399

Page seeded by OCA on Mon Jul 28 04:53:09 2008

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