1q0t

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[[Image:1q0t.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1q0t| PDB=1q0t | SCENE= }}
{{STRUCTURE_1q0t| PDB=1q0t | SCENE= }}
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'''Ternary Structure of T4DAM with AdoHcy and DNA'''
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===Ternary Structure of T4DAM with AdoHcy and DNA===
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==Overview==
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DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 12937411 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12937411}}
==About this Structure==
==About this Structure==
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[[Category: Methyltransferase]]
[[Category: Methyltransferase]]
[[Category: T4dam]]
[[Category: T4dam]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:43:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:58:38 2008''

Revision as of 01:58, 28 July 2008

Image:1q0t.png

Template:STRUCTURE 1q0t

Ternary Structure of T4DAM with AdoHcy and DNA

Template:ABSTRACT PUBMED 12937411

About this Structure

1Q0T is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Structure of the bacteriophage T4 DNA adenine methyltransferase., Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X, Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:12937411

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