2q80

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{{STRUCTURE_2q80| PDB=2q80 | SCENE= }}
{{STRUCTURE_2q80| PDB=2q80 | SCENE= }}
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'''Crystal structure of human geranylgeranyl pyrophosphate synthase bound to GGPP'''
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===Crystal structure of human geranylgeranyl pyrophosphate synthase bound to GGPP===
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==Overview==
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Modification of GTPases with isoprenoid molecules derived from geranylgeranyl pyrophosphate or farnesyl pyrophosphate is an essential requisite for cellular signaling pathways. The synthesis of these isoprenoids proceeds in mammals through the mevalonate pathway, and the final steps in the synthesis are catalyzed by the related enzymes farnesyl pyrophosphate synthase and geranylgeranyl pyrophosphate synthase. Both enzymes play crucial roles in cell survival, and inhibition of farnesyl pyrophosphate synthase by nitrogen-containing bisphosphonates is an established concept in the treatment of bone disorders such as osteoporosis or certain forms of cancer in bone. Here we report the crystal structure of human geranylgeranyl pyrophosphate synthase, the first mammalian ortholog to have its x-ray structure determined. It reveals that three dimers join together to form a propeller-bladed hexameric molecule with a mass of approximately 200 kDa. Structure-based sequence alignments predict this quaternary structure to be restricted to mammalian and insect orthologs, whereas fungal, bacterial, archaeal, and plant forms exhibit the dimeric organization also observed in farnesyl pyrophosphate synthase. Geranylgeranyl pyrophosphate derived from heterologous bacterial expression is tightly bound in a cavity distinct from the chain elongation site described for farnesyl pyrophosphate synthase. The structure most likely represents an inhibitory complex, which is further corroborated by steady-state kinetics, suggesting a possible feedback mechanism for regulating enzyme activity. Structural comparisons between members of this enzyme class give deeper insights into conserved features important for catalysis.
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==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Structural genomics consortium]]
[[Category: Structural genomics consortium]]
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Revision as of 02:12, 28 July 2008

Image:2q80.png

Template:STRUCTURE 2q80

Crystal structure of human geranylgeranyl pyrophosphate synthase bound to GGPP

Template:ABSTRACT PUBMED 16698791

About this Structure

2Q80 is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 2fvi. Full crystallographic information is available from OCA.

Reference

The crystal structure of human geranylgeranyl pyrophosphate synthase reveals a novel hexameric arrangement and inhibitory product binding., Kavanagh KL, Dunford JE, Bunkoczi G, Russell RG, Oppermann U, J Biol Chem. 2006 Aug 4;281(31):22004-12. Epub 2006 May 11. PMID:16698791

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