1ypv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ypv.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1ypv.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ypv| PDB=1ypv | SCENE= }}
{{STRUCTURE_1ypv| PDB=1ypv | SCENE= }}
-
'''Structure of human thymidylate synthase at low salt conditions'''
+
===Structure of human thymidylate synthase at low salt conditions===
-
==Overview==
+
<!--
-
Human thymidylate synthase, a target in cancer chemotherapy, was crystallized from PEG 3350 with 30 mM ammonium sulfate (AS) in the crystallization medium. The crystals are isomorphous with the high-salt crystals ( approximately 2.0 M AS) and the structure has been solved and refined (R = 22.6%, R(free) = 24.3%) at 1.8 A resolution. The high- and low-AS-concentration structures are quite similar, with loop 181-197 is in the inactive conformation. Also, residues 95-106 and 129-135 (eukaryotic inserts region) show high mobility as assessed by poor electron density and high values of crystallographic temperature factors (residues 1-25 and 108-129 are disordered in both structures). The high mobility of this region may reflect the situation at physiological ionic strength. Of the four sulfate ions observed bound at 2.0 M AS, only two are present at 30 mM AS. The inactive conformation appears to be stabilized by the side chain of Val3 or a leucine residue from the disordered regions. The low-salt conditions of these crystals should be much more suitable for the study of thymidylate synthase inhibitors, especially those that utilize sulfate-binding sites to stabilize the inactive conformation of loop 181-197.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15858273}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15858273 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15858273}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Methyltransferase]]
[[Category: Methyltransferase]]
[[Category: Thymidylate synthase]]
[[Category: Thymidylate synthase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:37:45 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:12:08 2008''

Revision as of 02:12, 28 July 2008

Image:1ypv.png

Template:STRUCTURE 1ypv

Structure of human thymidylate synthase at low salt conditions

Template:ABSTRACT PUBMED 15858273

About this Structure

1YPV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of human thymidylate synthase under low-salt conditions., Lovelace LL, Minor W, Lebioda L, Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):622-7. Epub 2005, Apr 20. PMID:15858273

Page seeded by OCA on Mon Jul 28 05:12:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ypv"
Personal tools