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| - | [[Image:1yuh.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:1yuh.png|left|200px]] |
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| | {{STRUCTURE_1yuh| PDB=1yuh | SCENE= }} | | {{STRUCTURE_1yuh| PDB=1yuh | SCENE= }} |
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| - | '''FAB FRAGMENT'''
| + | ===FAB FRAGMENT=== |
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| - | ==Overview==
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| - | Affinity maturation of the immune response to nitrophenol-containing antigens has been extensively investigated. Significant strides made during the past several years with the advent of PCR technology have provided a wealth of biochemical knowledge. Structural investigations of the phenomena have however been limited. We have determined the three-dimensional structure of the Fab fragment of 88C6/12, an anti-4-hydroxy-3-nitrophenyl acetic acid antibody complexed with the immunizing hapten and with a heteroclitic iodinated hapten. The crystallographic structure of the complexes reveals that the binding is stabilized by a number of hydrogen bonds and extensive van der Waals interactions between the hapten and the antibody. In addition, the Fab binding pocket contains a region of positive electrostatic potential well suited for interaction with the predominant resonance form of the nitrophenyl ring system. The observed heteroclicity towards the iodinated hapten is not a direct result of iodine-protein interactions, but results from the enhanced stability in the iodinated ring of the resonance form that binds the antibody. In addition this investigation provides a rationale for the strong preference for the substitution in the heavy chain from the germ-line gene encoded Trp 33 to Leu 33 in the mature anti-nitrophenol response.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8544863}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8544863 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8544863}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Immunoglobulin]] | | [[Category: Immunoglobulin]] |
| | [[Category: Lambda light chain]] | | [[Category: Lambda light chain]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:48:23 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:16:11 2008'' |
Revision as of 02:16, 28 July 2008
Template:STRUCTURE 1yuh
FAB FRAGMENT
Template:ABSTRACT PUBMED 8544863
About this Structure
1YUH is a Protein complex structure. Full crystallographic information is available from OCA.
Reference
Structural analysis of affinity maturation: the three-dimensional structures of complexes of an anti-nitrophenol antibody., Yuhasz SC, Parry C, Strand M, Amzel LM, Mol Immunol. 1995 Oct;32(14-15):1143-55. PMID:8544863
Page seeded by OCA on Mon Jul 28 05:16:11 2008
