1lkb
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(New page: 200px<br /><applet load="1lkb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lkb, resolution 1.70Å" /> '''Porcine Pancreatic E...)
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Revision as of 18:30, 20 November 2007
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Porcine Pancreatic Elastase/Na-Complex
Overview
Porcine pancreatic elastase has been crystallized at slightly acidic pH, under two similar but slightly different conditions. Diffraction data were, collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both, difference electron-density maps and anomalous difference electron-density, maps suggest that in crystals grown from a sodium sulfate solution PPE, binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in, crystals grown from a solution containing sodium citrate and calcium, chloride. This observation is in contradiction to most PPE structures, reported in the PDB. In addition to the metal-binding site, up to three, other binding sites, which appear to be anion-binding sites, could be, identified based on the observed anomalous intensity differences.
About this Structure
1LKB is a Single protein structure of sequence from Sus scrofa with SO4, CL and NA as ligands. Active as Pancreatic elastase, with EC number 3.4.21.36 Full crystallographic information is available from OCA.
Reference
Metal binding to porcine pancreatic elastase: calcium or not calcium., Weiss MS, Panjikar S, Nowak E, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1407-12. Epub 2002, Aug 23. PMID:12198296
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