2fd2

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{{STRUCTURE_2fd2| PDB=2fd2 | SCENE= }}
{{STRUCTURE_2fd2| PDB=2fd2 | SCENE= }}
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'''CRYSTALLOGRAPHIC ANALYSIS OF TWO SITE-DIRECTED MUTANTS OF AZOTOBACTER VINELANDII FERREDOXIN'''
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===CRYSTALLOGRAPHIC ANALYSIS OF TWO SITE-DIRECTED MUTANTS OF AZOTOBACTER VINELANDII FERREDOXIN===
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==Overview==
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The crystal structure of the C24A mutant of Azotobacter vinelandii 7Fe ferredoxin (FdI) has been solved and refined at 2.0-A resolution. The structure is isomorphous to native FdI except at the site of mutation where A24 moves toward the [4Fe-4S] cluster. In spite of this inefficient packing results: three of five van der Waals contacts from the S gamma of C24 in native FdI are lost and the remaining two become longer. Consequently, the [4Fe-4S] cluster is either disordered or has a higher temperature factor (B factor) compared to the rest of the C24A FdI molecule. In addition, the entire C24A FdI structure has a higher overall B factor than native FdI. Therefore, in comparison to native FdI, the C24A mutant is isomorphous but exhibits large differences in B factor, especially at the [4Fe-4S] cluster. In contrast, the C20A FdI structure (Martin, A. G., Burgess, B. K., Stout, C. D., Cash, V. L., Dean, D. R., Jensen, G. M., and Stephens, P. J. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 598-602), which contains large structural rearrangements in the vicinity of the [4Fe-4S] cluster, exhibits essentially no change in B factor. The conformational change observed at residue 24 is similar in both C24A and C20A FdI structures. The solvent accessibility of the Fe atoms in the [3Fe-4S] and [4Fe-4S] clusters is similar in C24A, C20A, and native FdI.
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(as it appears on PubMed at http://www.pubmed.gov), where 1939185 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1939185}}
==About this Structure==
==About this Structure==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Stout, C D.]]
[[Category: Stout, C D.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:45:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:17:28 2008''

Revision as of 02:17, 28 July 2008

Image:2fd2.png

Template:STRUCTURE 2fd2

CRYSTALLOGRAPHIC ANALYSIS OF TWO SITE-DIRECTED MUTANTS OF AZOTOBACTER VINELANDII FERREDOXIN

Template:ABSTRACT PUBMED 1939185

About this Structure

2FD2 is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.

Reference

Crystallographic analysis of two site-directed mutants of Azotobacter vinelandii ferredoxin., Soman J, Iismaa S, Stout CD, J Biol Chem. 1991 Nov 15;266(32):21558-62. PMID:1939185

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