1tu2

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[[Image:1tu2.gif|left|200px]]
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{{STRUCTURE_1tu2| PDB=1tu2 | SCENE= }}
{{STRUCTURE_1tu2| PDB=1tu2 | SCENE= }}
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'''THE COMPLEX OF NOSTOC CYTOCHROME F AND PLASTOCYANIN DETERMIN WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES'''
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===THE COMPLEX OF NOSTOC CYTOCHROME F AND PLASTOCYANIN DETERMIN WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES===
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==Overview==
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The complex between cytochrome f and plastocyanin from the cyanobacterium Nostoc has been characterized by NMR spectroscopy. The binding constant is 16 mM(-1), and the lifetime of the complex is much less than 10 ms. Intermolecular pseudo-contact shifts observed for the plastocyanin amide nuclei, caused by the heme iron, as well as the chemical-shift perturbation data were used as the sole experimental restraints to determine the orientation of plastocyanin relative to cytochrome f with a precision of 1.3 angstroms. The data show that the hydrophobic patch surrounding tyrosine 1 in cytochrome f docks the hydrophobic patch of plastocyanin. Charge complementarities are found between the rims of the respective recognition sites of cytochrome f and plastocyanin. Significant differences in the relative orientation of both proteins are found between this complex and those previously reported for plants and Phormidium, indicating that electrostatic and hydrophobic interactions are balanced differently in these complexes.
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The line below this paragraph, {{ABSTRACT_PUBMED_15705583}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15705583 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15705583}}
==About this Structure==
==About this Structure==
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1TU2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TU2 OCA].
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1TU2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TU2 OCA].
==Reference==
==Reference==
Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. PCC 7119 as determined by paramagnetic NMR. The balance between electrostatic and hydrophobic interactions within the transient complex determines the relative orientation of the two proteins., Diaz-Moreno I, Diaz-Quintana A, De la Rosa MA, Ubbink M, J Biol Chem. 2005 May 13;280(19):18908-15. Epub 2005 Feb 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15705583 15705583]
Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. PCC 7119 as determined by paramagnetic NMR. The balance between electrostatic and hydrophobic interactions within the transient complex determines the relative orientation of the two proteins., Diaz-Moreno I, Diaz-Quintana A, De la Rosa MA, Ubbink M, J Biol Chem. 2005 May 13;280(19):18908-15. Epub 2005 Feb 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15705583 15705583]
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Cloning and correct expression in Escherichia coli of the petE and petJ genes respectively encoding plastocyanin and cytochrome c6 from the cyanobacterium Anabaena sp. PCC 7119., Molina-Heredia FP, Hervas M, Navarro JA, De la Rosa MA, Biochem Biophys Res Commun. 1998 Feb 4;243(1):302-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9473522 9473522]
[[Category: Anabaena sp.]]
[[Category: Anabaena sp.]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
[[Category: Pseudocontact shift]]
[[Category: Pseudocontact shift]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:21:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:18:06 2008''

Revision as of 02:18, 28 July 2008

Image:1tu2.png

Template:STRUCTURE 1tu2

THE COMPLEX OF NOSTOC CYTOCHROME F AND PLASTOCYANIN DETERMIN WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES

Template:ABSTRACT PUBMED 15705583

About this Structure

1TU2 is a Protein complex structure of sequences from Anabaena sp.. Full experimental information is available from OCA.

Reference

Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. PCC 7119 as determined by paramagnetic NMR. The balance between electrostatic and hydrophobic interactions within the transient complex determines the relative orientation of the two proteins., Diaz-Moreno I, Diaz-Quintana A, De la Rosa MA, Ubbink M, J Biol Chem. 2005 May 13;280(19):18908-15. Epub 2005 Feb 10. PMID:15705583

Cloning and correct expression in Escherichia coli of the petE and petJ genes respectively encoding plastocyanin and cytochrome c6 from the cyanobacterium Anabaena sp. PCC 7119., Molina-Heredia FP, Hervas M, Navarro JA, De la Rosa MA, Biochem Biophys Res Commun. 1998 Feb 4;243(1):302-6. PMID:9473522

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