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| - | [[Image:2pam.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2pam.png|left|200px]] |
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| | {{STRUCTURE_2pam| PDB=2pam | SCENE= }} | | {{STRUCTURE_2pam| PDB=2pam | SCENE= }} |
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| - | '''Structure of a H49N, H51N double mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP'''
| + | ===Structure of a H49N, H51N double mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP=== |
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| - | ==Overview==
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| - | The repeating unit of the glycan chain in the S-layer of the bacterium Aneurinibacillus thermoaerophilus L420-91(T) is composed of four alpha-d-rhamnose molecules and two 3-acetamido-3,6-dideoxy-alpha-d-galactose moieties (abbreviated as Fucp3NAc). Formation of the glycan layer requires nucleotide-activated sugars as the donor molecules. Whereas the enzymes involved in the synthesis of GDP-rhamnose have been well characterized, less is known regarding the structures and enzymatic mechanisms of the enzymes required for the production of dTDP-Fucp3NAc. One of the enzymes involved in the biosynthesis of dTDP-Fucp3NAc is a 3,4-ketoisomerase, hereafter referred to as FdtA. Here we describe the first three-dimensional structure of this sugar isomerase complexed with dTDP and solved to 1.5 A resolution. The FdtA dimer assumes an almost jellyfish-like appearance with the sole alpha-helices representing the tentacles. Formation of the FdtA dimer represents a classical example of domain swapping whereby beta-strands 2 and 3 from one subunit form part of a beta-sheet in the second subunit. The active site architecture of FdtA is characterized by a cluster of three histidine residues, two of which, His(49) and His(51), appear to be strictly conserved in the amino acid sequences deposited to date. Site-directed mutagenesis experiments, enzymatic assays, and x-ray crystallographic analyses suggest that His(49) functions as an active site base. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17459872}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17459872 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17459872}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ketoisomerase]] | | [[Category: Ketoisomerase]] |
| | [[Category: S-layer biosynthesis]] | | [[Category: S-layer biosynthesis]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:44:18 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:21:20 2008'' |
Revision as of 02:21, 28 July 2008
Template:STRUCTURE 2pam
Structure of a H49N, H51N double mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP
Template:ABSTRACT PUBMED 17459872
About this Structure
2PAM is a Single protein structure of sequence from Aneurinibacillus thermoaerophilus. Full crystallographic information is available from OCA.
Reference
The x-ray structure of dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase., Davis ML, Thoden JB, Holden HM, J Biol Chem. 2007 Jun 29;282(26):19227-36. Epub 2007 Apr 25. PMID:17459872
Page seeded by OCA on Mon Jul 28 05:21:20 2008
