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| | {{STRUCTURE_251d| PDB=251d | SCENE= }} | | {{STRUCTURE_251d| PDB=251d | SCENE= }} |
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| - | '''SINGLE CRYSTAL AND MOLECULAR STRUCTURE OF D(CTCGAG)'''
| + | ===SINGLE CRYSTAL AND MOLECULAR STRUCTURE OF D(CTCGAG)=== |
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| - | ==Overview==
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| - | The crystal structure of the B-DNA hexamer d(CTCGAG) has been solved at 1.9 A resolution by iterative single isomorphous replacement, using the brominated derivative d(CG5BrCGAG), and refined to an R-factor of 18.6% for 120 nonhydrogen nucleic acid atoms and 32 water molecules. Although the central four base pairs form a typical B-form helix, several parameters suggest a transition to an A-like conformation at the termini. Based on this observation, a B-to-A transition was modeled, maintaining efficient base stacking across the junction. The wide minor groove (approximately 6.9 A) is reminiscent of that in the side-by-side double drug-DNA complexes and hosts a double spine of hydration. The global helix axes of the pseudo-continuous helices are at an acute angle of 60 degrees. The pseudocontinuous stacking is reinforced by the minor groove water structure extending between the two duplexes. The crossover point of two pairs of stacked duplexes is at the stacking junction, unlike that observed in the B-DNA decamers and dodecamers. This arrangement may have implications for the structure of a four-way DNA junction. The duplexes are arranged around a large (approximately 20 A diameter) channel centered on a 6(2) screw axis. | + | The line below this paragraph, {{ABSTRACT_PUBMED_8744323}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8744323 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8744323}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: B-dna]] | | [[Category: B-dna]] |
| | [[Category: Double helix]] | | [[Category: Double helix]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:23:09 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:24:46 2008'' |
Revision as of 02:24, 28 July 2008
Template:STRUCTURE 251d
SINGLE CRYSTAL AND MOLECULAR STRUCTURE OF D(CTCGAG)
Template:ABSTRACT PUBMED 8744323
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of the B-DNA hexamer d(CTCGAG): model for an A-to-B transition., Wahl MC, Rao ST, Sundaralingam M, Biophys J. 1996 Jun;70(6):2857-66. PMID:8744323
Page seeded by OCA on Mon Jul 28 05:24:46 2008
