1rxi

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{{STRUCTURE_1rxi| PDB=1rxi | SCENE= }}
{{STRUCTURE_1rxi| PDB=1rxi | SCENE= }}
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'''pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S'''
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===pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S===
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==Overview==
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Structural insights into formation of the complex between the ubiquitous thiol-disulfide oxidoreductase thioredoxin and its oxidized substrate are under-documented owing to its entropical instability. In vitro, it is possible via a reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) to make a stable mixed-disulfide complex between thioredoxin from Staphylococcus aureus and one of its substrates, oxidized pI258 arsenate reductase (ArsC) from S. aureus. In the absence of the crystal structure of an ArsC-thioredoxin complex, the structures of two precursors of the complex, the ArsC triple mutant ArsC C10SC15AC82S and its 5-thio-2-nitrobenzoic acid (TNB) adduct, were determined. The ArsC triple mutant has a structure very similar to that of the reduced form of wild-type ArsC, with a folded redox helix and a buried catalytic Cys89. In the adduct form, the TNB molecule is buried in a hydrophobic pocket and the disulfide bridge between TNB and Cys89 is sterically inaccessible to thioredoxin. In order to form a mixed disulfide between ArsC and thioredoxin, a change in the orientation of the TNB-Cys89 disulfide in the structure is necessary.
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(as it appears on PubMed at http://www.pubmed.gov), where 15159594 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15159594}}
==About this Structure==
==About this Structure==
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[[Category: Wyns, L.]]
[[Category: Wyns, L.]]
[[Category: Arsc]]
[[Category: Arsc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:28:02 2008''

Revision as of 02:28, 28 July 2008

Image:1rxi.png

Template:STRUCTURE 1rxi

pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S

Template:ABSTRACT PUBMED 15159594

About this Structure

1RXI is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct., Messens J, Van Molle I, Vanhaesebrouck P, Van Belle K, Wahni K, Martins JC, Wyns L, Loris R, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1180-4. Epub 2004, May 21. PMID:15159594

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