1rjl

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{{STRUCTURE_1rjl| PDB=1rjl | SCENE= }}
{{STRUCTURE_1rjl| PDB=1rjl | SCENE= }}
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'''Structure of the complex between OspB-CT and bactericidal Fab-H6831'''
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===Structure of the complex between OspB-CT and bactericidal Fab-H6831===
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==Overview==
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Certain antibody Fab fragments directed against the C terminus of outer surface protein B (OspB), a major lipoprotein of the Lyme disease spirochete, Borrelia burgdorferi, have the unusual property of being bactericidal even in the absence of complement. We report here x-ray crystal structures of a C-terminal fragment of B. burgdorferi OspB, which spans residues 152-296, alone at 2.0-A resolution, and in a complex with the bactericidal Fab H6831 at 2.6-A resolution. The H6831 epitope is topologically analogous to the LA-2 epitope of OspA and is centered around OspB Lys-253, a residue essential for H6831 recognition. A beta-sheet present in the free OspB fragment is either disordered or removed by proteolysis in the H6831-bound complex. Other conformational changes between free and H6831-bound structures are minor and appear to be related to this loss. In both crystal structures, OspB C-terminal fragments form artificial dimers connected by intermolecular beta-sheets. OspB structure, stability, and possible mechanisms of killing by H6831 and other bactericidal Fabs are discussed in light of the structural data.
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(as it appears on PubMed at http://www.pubmed.gov), where 15713683 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15713683}}
==About this Structure==
==About this Structure==
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[[Category: Antibody-protein complex]]
[[Category: Antibody-protein complex]]
[[Category: Beta sheet]]
[[Category: Beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:34:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:37:01 2008''

Revision as of 02:37, 28 July 2008

Image:1rjl.png


PDB ID 1rjl

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1rjl, resolution 2.60Å ()
Non-Standard Residues:
Gene: OSPB, BBA16 (Borrelia burgdorferi)
Related: 1p4p, 1fj1, 1osp
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Structure of the complex between OspB-CT and bactericidal Fab-H6831

Template:ABSTRACT PUBMED 15713683

About this Structure

1RJL is a Single protein structure of sequence from Borrelia burgdorferi and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural investigation of Borrelia burgdorferi OspB, a bactericidal Fab target., Becker M, Bunikis J, Lade BD, Dunn JJ, Barbour AG, Lawson CL, J Biol Chem. 2005 Apr 29;280(17):17363-70. Epub 2005 Feb 15. PMID:15713683

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