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1qsw

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{{STRUCTURE_1qsw| PDB=1qsw | SCENE= }}
{{STRUCTURE_1qsw| PDB=1qsw | SCENE= }}
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'''CRYSTAL STRUCTURE ANALYSIS OF A HUMAN LYSOZYME MUTANT W64C C65A'''
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===CRYSTAL STRUCTURE ANALYSIS OF A HUMAN LYSOZYME MUTANT W64C C65A===
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==Overview==
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The three-dimensional structure of a mutant human lysozyme, W64CC65A, in which a non-native disulfide bond Cys64--Cys81 is substituted for the Cys65--Cys81 of the wild type protein by replacing Trp64 and Cys65 with Cys and Ala, respectively, was determined by X-ray crystallography and refined to an R-value of 0.181, using 33,187 reflections at 1.87-A resolution. The refined model of the W64CC65A protein consisted of four molecules, which were related by two noncrystallographic twofold axes and a translation vector. Although no specific structural differences could be observed among these four molecules, the overall B-factors of each molecule were quite different. The overall structure of W64CC65A, especially in the alpha-helical domain, was found to be quite similar to that of the wild type protein. Moreover, the side-chain conformation of the newly formed Cys64--Cys81 bond was quite similar to that of the Cys65--Cys81 bond of the wild-type protein. However, in the beta-sheet domain, the main-chain atoms of the loop region from positions 66-75 could not be determined, and significant structural changes due to the formation of the non-native disulfide bond could be observed. From these results, it is clear that the loop region of the mutant protein does not fold with the specific folding as observed in the wild-type protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 11340658 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11340658}}
==About this Structure==
==About this Structure==
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[[Category: Miki, K.]]
[[Category: Miki, K.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:40:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:40:25 2008''

Revision as of 02:40, 28 July 2008

Image:1qsw.png

Template:STRUCTURE 1qsw

CRYSTAL STRUCTURE ANALYSIS OF A HUMAN LYSOZYME MUTANT W64C C65A

Template:ABSTRACT PUBMED 11340658

About this Structure

1QSW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of a mutant human lysozyme with a substituted disulfide bond., Inaka K, Kanaya E, Kikuchi M, Miki K, Proteins. 2001 Jun 1;43(4):413-9. PMID:11340658

Page seeded by OCA on Mon Jul 28 05:40:25 2008

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