This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2aqj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2aqj.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2aqj.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2aqj| PDB=2aqj | SCENE= }}
{{STRUCTURE_2aqj| PDB=2aqj | SCENE= }}
-
'''The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination'''
+
===The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination===
-
==Overview==
+
<!--
-
Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16195462}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16195462 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16195462}}
==About this Structure==
==About this Structure==
Line 20: Line 24:
==Reference==
==Reference==
Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16195462 16195462]
Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16195462 16195462]
 +
 +
Crystallization and X-ray diffraction of a halogenating enzyme, tryptophan 7-halogenase, from Pseudomonas fluorescens., Dong C, Kotzsch A, Dorward M, van Pee KH, Naismith JH, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1438-40. Epub 2004, Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272170 15272170]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 36: Line 42:
[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Tryptophan 7-halogenase]]
[[Category: Tryptophan 7-halogenase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:21:03 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:41:49 2008''

Revision as of 02:41, 28 July 2008

Image:2aqj.png

Template:STRUCTURE 2aqj

The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination

Template:ABSTRACT PUBMED 16195462

About this Structure

2AQJ is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462

Crystallization and X-ray diffraction of a halogenating enzyme, tryptophan 7-halogenase, from Pseudomonas fluorescens., Dong C, Kotzsch A, Dorward M, van Pee KH, Naismith JH, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1438-40. Epub 2004, Jul 21. PMID:15272170

Page seeded by OCA on Mon Jul 28 05:41:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2aqj"
Personal tools