2a41

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{{STRUCTURE_2a41| PDB=2a41 | SCENE= }}
{{STRUCTURE_2a41| PDB=2a41 | SCENE= }}
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'''Ternary complex of the WH2 Domain of WIP with Actin-DNAse I'''
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===Ternary complex of the WH2 Domain of WIP with Actin-DNAse I===
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==Overview==
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Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) is a small and widespread actin-binding motif. In the WASP family, WH2 plays a role in filament nucleation by Arp2/3 complex. Here we describe the crystal structures of complexes of actin with the WH2 domains of WASP, WASP-family verprolin homologous protein, and WASP-interacting protein. Despite low sequence identity, WH2 shares structural similarity with the N-terminal portion of the actin monomer-sequestering thymosin beta domain (Tbeta). We show that both domains inhibit nucleotide exchange by targeting the cleft between actin subdomains 1 and 3, a common binding site for many unrelated actin-binding proteins. Importantly, WH2 is significantly shorter than Tbeta but binds actin with approximately 10-fold higher affinity. WH2 lacks a C-terminal extension that in Tbeta4 becomes involved in monomer sequestration by interfering with intersubunit contacts in F-actin. Owing to their shorter length, WH2 domains connected in tandem by short linkers can coexist with intersubunit contacts in F-actin and are proposed to function in filament nucleation by lining up actin subunits along a filament strand. The WH2-central region of WASP-family proteins is proposed to function in an analogous way by forming a special class of tandem repeats whose function is to line up actin and Arp2 during Arp2/3 nucleation. The structures also suggest a mechanism for how profilin-binding Pro-rich sequences positioned N-terminal to WH2 could feed actin monomers directly to WH2, thereby playing a role in filament elongation.
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(as it appears on PubMed at http://www.pubmed.gov), where 16275905 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16275905}}
==About this Structure==
==About this Structure==
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[[Category: Wh2]]
[[Category: Wh2]]
[[Category: Wip]]
[[Category: Wip]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:45:23 2008''

Revision as of 02:45, 28 July 2008

Image:2a41.png

Template:STRUCTURE 2a41

Ternary complex of the WH2 Domain of WIP with Actin-DNAse I

Template:ABSTRACT PUBMED 16275905

About this Structure

2A41 is a Protein complex structure of sequences from Bos taurus and Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly., Chereau D, Kerff F, Graceffa P, Grabarek Z, Langsetmo K, Dominguez R, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16644-9. Epub 2005 Nov 7. PMID:16275905

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