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| - | [[Image:1umj.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1umj| PDB=1umj | SCENE= }} | | {{STRUCTURE_1umj| PDB=1umj | SCENE= }} |
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| - | '''Crystal structure of Pyrococcus horikoshii CutA in the presence of 3M guanidine hydrochloride'''
| + | ===Crystal structure of Pyrococcus horikoshii CutA in the presence of 3M guanidine hydrochloride=== |
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| - | ==Overview==
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| - | This study was carried out to investigate the structural perturbation of the protein's local structure by the denaturants under non-denaturing conditions. Crystal structure of CutA from an archaeon Pyrococcus horikosii (PhoCutA), a heavy-metal binding protein, was determined at 1.6-angstroms resolution in the presence of 3 M guanidine HCl (GdnHCl). Native PhoCutA has a large number of short intramolecular hydrogen bonds and salt bridges on the protein surface, of which greater than 90% of hydrogen bonds and all salt bridges were retained in 3 M GdnHCl. Hydrogen bonds that disappeared in the GdnHCl crystal structure were mainly located on the protein surface, especially around the structurally perturbed loop, suggesting interactions between peptide groups and GdnHCl. Only a few GdnH+ ions were observed in the crystal structure, although none at the surface, of the protein. Two GdnH+ ions were observed in the center of the trimeric structure, replacing water molecules, and were hydrogen bonded with Asp84 and Asp86 of each chain. The exterior loop from Tyr39 to Lys44, including Trp40-Trp41, was perturbed structurally. Decreases in temperature factors were observed in beta strand 5 and the N terminus of helix 3. These results suggest the specific bindings of GdnH+ with some acidic residues and the non-specific bindings around Trp residues and peptide groups on the protein surface and that binding of GdnHCl to the native protein is limited, resulting in local structural perturbation.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15351719}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15351719 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15351719}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Cuta]] | | [[Category: Cuta]] |
| | [[Category: Structural genomic]] | | [[Category: Structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:25:40 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:46:01 2008'' |
Revision as of 02:46, 28 July 2008
Template:STRUCTURE 1umj
Crystal structure of Pyrococcus horikoshii CutA in the presence of 3M guanidine hydrochloride
Template:ABSTRACT PUBMED 15351719
About this Structure
1UMJ is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.
Reference
Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride., Tanaka Y, Tsumoto K, Umetsu M, Nakanishi T, Yasutake Y, Sakai N, Yao M, Tanaka I, Arakawa T, Kumagai I, Biochem Biophys Res Commun. 2004 Oct 8;323(1):185-91. PMID:15351719
Page seeded by OCA on Mon Jul 28 05:46:01 2008
