1uvk

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[[Image:1uvk.gif|left|200px]]
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{{Seed}}
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[[Image:1uvk.png|left|200px]]
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{{STRUCTURE_1uvk| PDB=1uvk | SCENE= }}
{{STRUCTURE_1uvk| PDB=1uvk | SCENE= }}
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'''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX'''
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===THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX===
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==Overview==
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The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+.
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The line below this paragraph, {{ABSTRACT_PUBMED_14962391}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14962391 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14962391}}
==About this Structure==
==About this Structure==
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[[Category: Polymerase]]
[[Category: Polymerase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Rna-dependent rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:56:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:48:03 2008''

Revision as of 02:48, 28 July 2008

Image:1uvk.png

Template:STRUCTURE 1uvk

THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX

Template:ABSTRACT PUBMED 14962391

About this Structure

1UVK is a Protein complex structure of sequences from Pseudomonas phage phi6. Full crystallographic information is available from OCA.

Reference

The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase., Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM, Structure. 2004 Feb;12(2):307-16. PMID:14962391

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