1lnl

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Revision as of 18:35, 20 November 2007

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spinqualitylabelsall models 1lnl, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structure of deoxygenated hemocyanin from Rapana thomasiana

Overview

Structure-function relationships in a molluscan hemocyanin have been, investigated by determining the crystal structure of the Rapana thomasiana, (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38, A resolution. This is the first X-ray structure of an unit from the wall, of the molluscan hemocyanin cylinder. The crystal structure of RtH2e, demonstrates molecular self-assembly of six identical molecules forming a, regular hexameric cylinder. This suggests how the functional units are, ordered in the wall of the native molluscan hemocyanins. The molecular, arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of, the cylinders. A molecular mechanism for cooperative dioxygen binding in, molluscan hemocyanins is proposed on the basis of the molecular, interactions between the protomers. In particular, the deoxygenated RtH2e, structure reveals a tunnel leading from two opposite sides of the molecule, to the active site. The tunnel represents a possible entrance pathway for, dioxygen molecules. No such tunnels have been observed in the crystal, structure of the oxy-Odg, a functional unit from the Octopus dofleini, (cephalopod) hemocyanin in oxygenated form.

About this Structure

1LNL is a Single protein structure of sequence from Rapana thomasiana with NAG and CU as ligands. Full crystallographic information is available from OCA.

Reference

The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity., Perbandt M, Guthohrlein EW, Rypniewski W, Idakieva K, Stoeva S, Voelter W, Genov N, Betzel C, Biochemistry. 2003 Jun 3;42(21):6341-6. PMID:12767214

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