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| | {{STRUCTURE_1zch| PDB=1zch | SCENE= }} | | {{STRUCTURE_1zch| PDB=1zch | SCENE= }} |
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| - | '''Structure of the hypothetical oxidoreductase YcnD from Bacillus subtilis'''
| + | ===Structure of the hypothetical oxidoreductase YcnD from Bacillus subtilis=== |
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| - | ==Overview==
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| - | YcnD from the gram-positive bacterium Bacillus subtilis is a member of a family of bacterial proteins that act as NADH- and/or NADPH-dependent oxidoreductases. Here, we report for the first time on the biochemical characterization of the purified protein, demonstrating that YcnD is an FMN-containing enzyme that can be reduced by NADH or NADPH (Km = 6.4 and 4.4 microM, respectively). In the presence of free FMN as the electron-accepting substrate, the latter reductant showed a ping-pong Bi-Bi reaction mechanism, whereas utilization of NADH is competitively inhibited by this substrate. This finding suggests that NADPH is the physiological reductant of the enzyme. We also show that YcnD reduces nitro-organic compounds, chromate, and a series of azo dyes. The reduction of azo dyes appears to be mediated by free reduced FMN because the reaction is considerably slower in its absence. Structure determination by X-ray crystallography revealed that YcnD folds into a three layer alpha-beta-alpha sandwich strongly resembling the topology of the NADH oxidase superfamily. Similar to homologous bacterial oxidoreductase, YcnD forms homodimers with an extended dimer interface. The biochemical data and the structure are discussed in light of the putative physiological function of YcnD as an oxidoreductase delivering reduced FMN to enzymes that require the reduced cofactor for activity.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16229462}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16229462 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16229462}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Nadh-oxidase]] | | [[Category: Nadh-oxidase]] |
| | [[Category: Nitroreductase]] | | [[Category: Nitroreductase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:27:40 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:55:24 2008'' |
Revision as of 02:55, 28 July 2008
Template:STRUCTURE 1zch
Structure of the hypothetical oxidoreductase YcnD from Bacillus subtilis
Template:ABSTRACT PUBMED 16229462
About this Structure
1ZCH is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Structure and function of YcnD from Bacillus subtilis, a flavin-containing oxidoreductase., Morokutti A, Lyskowski A, Sollner S, Pointner E, Fitzpatrick TB, Kratky C, Gruber K, Macheroux P, Biochemistry. 2005 Oct 25;44(42):13724-33. PMID:16229462
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