1yua

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[[Image:1yua.gif|left|200px]]
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{{Seed}}
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[[Image:1yua.png|left|200px]]
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{{STRUCTURE_1yua| PDB=1yua | SCENE= }}
{{STRUCTURE_1yua| PDB=1yua | SCENE= }}
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'''C-TERMINAL DOMAIN OF ESCHERICHIA COLI TOPOISOMERASE I'''
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===C-TERMINAL DOMAIN OF ESCHERICHIA COLI TOPOISOMERASE I===
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==Overview==
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Escherichia coli DNA topoisomerase I catalyzes the interconversion of different topological forms of DNA. In this paper we describe NMR studies of a 14K C-terminal fragment of this enzyme that binds preferentially to single-stranded DNA and enhances the enzyme's ability to relax negatively supercoiled DNA under high salt conditions. The 1H, 13C, and 15N resonances of the protein were assigned from a number of heteronuclear multidimensional NMR experiments, and the three-dimensional structure of the protein was determined from a total of 2188 NMR-derived restraints. The root-mean-square deviation about the mean coordinate positions for residues 13-120 is 0.68 +/- 0.11 A for the backbone atoms and 1.09 +/- 0.09 A for all heavy atoms. The overall fold, which consists of two four-stranded beta-sheets separated by two helices, differs from other DNA- and RNA-binding proteins such as gene 5, cold shock protein, and hnRNP C. From an analysis of the changes in chemical shift upon the addition of single-stranded DNA, the location of the oligonucleotide binding site was determined. The binding site consists of a beta-sheet containing positively charged and aromatic amino acids and, in spite of its different structure, is similar to that found in other proteins that bind single-stranded oligonucleotides.
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The line below this paragraph, {{ABSTRACT_PUBMED_7779808}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 7779808 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7779808}}
==About this Structure==
==About this Structure==
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1YUA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUA OCA].
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1YUA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUA OCA].
==Reference==
==Reference==
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[[Category: Zhu, C X.]]
[[Category: Zhu, C X.]]
[[Category: Gene-regulating protein]]
[[Category: Gene-regulating protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:47:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:57:16 2008''

Revision as of 02:57, 28 July 2008

Image:1yua.png

Template:STRUCTURE 1yua

C-TERMINAL DOMAIN OF ESCHERICHIA COLI TOPOISOMERASE I

Template:ABSTRACT PUBMED 7779808

About this Structure

1YUA is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Solution structure of the C-terminal single-stranded DNA-binding domain of Escherichia coli topoisomerase I., Yu L, Zhu CX, Tse-Dinh YC, Fesik SW, Biochemistry. 1995 Jun 13;34(23):7622-8. PMID:7779808

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