1xi5

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{{STRUCTURE_1xi5| PDB=1xi5 | SCENE= }}
{{STRUCTURE_1xi5| PDB=1xi5 | SCENE= }}
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'''Clathrin D6 coat with auxilin J-domain'''
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===Clathrin D6 coat with auxilin J-domain===
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==Overview==
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Clathrin-coated pits invaginate from specific membrane compartments and pinch off as coated vesicles. These vesicles then uncoat rapidly once released. The Hsc70 molecular chaperone effects the uncoating reaction, and is guided to appropriate locations on clathrin lattices by the J-domain-containing co-chaperone molecule auxilin. This raises the question of how a local event such as ATP hydrolysis by Hsc70 can catalyse a global disassembly. Here, we have used electron cryomicroscopy to determine 12-A-resolution structures of in-vitro-assembled clathrin coats in association with a carboxy-terminal fragment of auxilin that contains both the clathrin-binding region and the J domain. We have located the auxilin fragment by computing differences between these structures and those lacking auxilin (described in an accompanying paper). Auxilin binds within the clathrin lattice near contacts between an inward-projecting C-terminal helical tripod and the crossing of two 'ankle' segments; it also contacts the terminal domain of yet another clathrin 'leg'. It therefore recruits Hsc70 to the neighbourhood of a set of critical interactions. Auxilin binding produces a local change in heavy-chain contacts, creating a detectable global distortion of the clathrin coat. We propose a mechanism by which local destabilization of the lattice promotes general uncoating.
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The line below this paragraph, {{ABSTRACT_PUBMED_15502813}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15502813 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15502813}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating., Fotin A, Cheng Y, Grigorieff N, Walz T, Harrison SC, Kirchhausen T, Nature. 2004 Dec 2;432(7017):649-53. Epub 2004 Oct 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502813 15502813]
Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating., Fotin A, Cheng Y, Grigorieff N, Walz T, Harrison SC, Kirchhausen T, Nature. 2004 Dec 2;432(7017):649-53. Epub 2004 Oct 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502813 15502813]
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Molecular model for a complete clathrin lattice from electron cryomicroscopy., Fotin A, Cheng Y, Sliz P, Grigorieff N, Harrison SC, Kirchhausen T, Walz T, Nature. 2004 Dec 2;432(7017):573-9. Epub 2004 Oct 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502812 15502812]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Beta-propeller]]
[[Category: Beta-propeller]]
[[Category: Clathrin]]
[[Category: Clathrin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:03:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:58:35 2008''

Revision as of 02:58, 28 July 2008

Image:1xi5.png

Template:STRUCTURE 1xi5

Clathrin D6 coat with auxilin J-domain

Template:ABSTRACT PUBMED 15502813

About this Structure

1XI5 is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating., Fotin A, Cheng Y, Grigorieff N, Walz T, Harrison SC, Kirchhausen T, Nature. 2004 Dec 2;432(7017):649-53. Epub 2004 Oct 24. PMID:15502813

Molecular model for a complete clathrin lattice from electron cryomicroscopy., Fotin A, Cheng Y, Sliz P, Grigorieff N, Harrison SC, Kirchhausen T, Walz T, Nature. 2004 Dec 2;432(7017):573-9. Epub 2004 Oct 24. PMID:15502812

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