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1rgf

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[[Image:1rgf.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1rgf| PDB=1rgf | SCENE= }}
{{STRUCTURE_1rgf| PDB=1rgf | SCENE= }}
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'''HYDROLASE, GUANYLORIBONUCLEASE'''
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===HYDROLASE, GUANYLORIBONUCLEASE===
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==Overview==
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Crystals of ribonuclease from Streptomyces aureofaciens diffract to atomic resolution at room temperature. Using synchrotron radiation and an imaging-plate scanner, X-ray data have been recorded to 1.20 A resolution from a crystal of native enzyme and to 1.15 A from a crystal of a complex with guanosine-2'-monophosphate. Refinement with anisotropic atomic temperature factors resulted in increased accuracy of the structure. The R factors for the two structures are 10.6 and 10.9%. The estimated r.m.s. error in the coordinates is 0.05 A, less than half that obtained in the previous analysis at 1.7 A resolution. For the well ordered part of the main chain the error falls to below 0.02 A as estimated from inversion of the least-squares matrix. The two independent molecules in the asymmetric unit allowed detailed analysis of peptide planarity and some torsion angles. The high accuracy of the analysis revealed density for a partially occupied anion in the nucleotide binding site of molecule A in the native structure which was not seen at lower resolution. The anisotropic model allowed correction of the identity of the residue at position 72 from cysteine to threonine. Cys72 SG had been modelled in previous analyses with two conformations. The solvent structure was modelled by means of an automated procedure employing a set of objective criteria. The solvent structure for models refined using different programs with isotropic and anisotropic description of thermal motion is compared.
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(as it appears on PubMed at http://www.pubmed.gov), where 15299705 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299705}}
==About this Structure==
==About this Structure==
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[[Category: Sevcik, J.]]
[[Category: Sevcik, J.]]
[[Category: Wilson, K S.]]
[[Category: Wilson, K S.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:27:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:05:20 2008''

Revision as of 03:05, 28 July 2008

Image:1rgf.png

Template:STRUCTURE 1rgf

HYDROLASE, GUANYLORIBONUCLEASE

Template:ABSTRACT PUBMED 15299705

About this Structure

1RGF is a Single protein structure of sequence from Streptomyces aureofaciens. Full crystallographic information is available from OCA.

Reference

Ribonuclease from Streptomyces aureofaciens at atomic resolution., Sevcik J, Dauter Z, Lamzin VS, Wilson KS, Acta Crystallogr D Biol Crystallogr. 1996 Mar 1;52(Pt 2):327-44. PMID:15299705

Page seeded by OCA on Mon Jul 28 06:05:20 2008

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