This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1nc2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1nc2.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1nc2.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1nc2| PDB=1nc2 | SCENE= }}
{{STRUCTURE_1nc2| PDB=1nc2 | SCENE= }}
-
'''Crystal Structure of Monoclonal Antibody 2D12.5 Fab Complexed with Y-DOTA'''
+
===Crystal Structure of Monoclonal Antibody 2D12.5 Fab Complexed with Y-DOTA===
-
==Overview==
+
<!--
-
We report the crystal structures of antibody 2D12.5 Fab bound to an yttrium-DOTA analogue and separately to a gadolinium-DOTA analogue. The rare earth elements have many useful properties as probes, and 2D12.5 binds the DOTA (1,4,7,10-tetraazacyclododecane-N,N',N' ',N' "-tetraacetic acid) complexes of all of them (Corneillie et al. J. Am. Chem. Soc. 2003, 125, 3436-3437). The structures show that there are no direct protein-metal interactions: a bridging water acts as a link between the protein and metal, with the chelate present as the M isomer in each case. DOTA forms an amphipathic cylinder with the charged carboxylate groups toward the face of the chelate near the metal ion, while nonpolar methylene groups from the macrocycle and the carboxymethyl groups occupy the rear and sides of the molecule. The orientation of the metal-DOTA in the 2D12.5 complex places most of the methylene carbon atoms of DOTA in hydrophobic contact with aromatic protein side chains. Other binding interactions between the metal complex and the antibody include a bidentate salt bridge, four direct H-bonds, and four to five water-mediated H-bonds. We find that 2D12.5 exhibits enantiomeric binding generality, binding yttrium chelates in both Lambda(deltadeltadeltadelta) and Delta(lambdalambdalambdalambda) configurations with modestly different affinities. This develops from the symmetrical nature of the DOTA chelate, which places heteroatoms and hydrophobic atoms in approximately the same relative positions regardless of the helicity of DOTA.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14653738}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14653738 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14653738}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Rare earth]]
[[Category: Rare earth]]
[[Category: Yttrium]]
[[Category: Yttrium]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:21:13 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:07:12 2008''

Revision as of 03:07, 28 July 2008

Image:1nc2.png

Template:STRUCTURE 1nc2

Crystal Structure of Monoclonal Antibody 2D12.5 Fab Complexed with Y-DOTA

Template:ABSTRACT PUBMED 14653738

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structures of two complexes of the rare-earth-DOTA-binding antibody 2D12.5: ligand generality from a chiral system., Corneillie TM, Fisher AJ, Meares CF, J Am Chem Soc. 2003 Dec 10;125(49):15039-48. PMID:14653738

Page seeded by OCA on Mon Jul 28 06:07:12 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nc2"
Personal tools