1vgr

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{{STRUCTURE_1vgr| PDB=1vgr | SCENE= }}
{{STRUCTURE_1vgr| PDB=1vgr | SCENE= }}
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'''Formyl-CoA transferase mutant Asp169 to Glu'''
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===Formyl-CoA transferase mutant Asp169 to Glu===
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==Overview==
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Oxalobacter formigenes is an obligate anaerobe that colonizes the human gastrointestinal tract and employs oxalate breakdown to generate ATP in a novel process involving the interplay of two coupled enzymes and a membrane-bound oxalate:formate antiporter. Formyl-CoA transferase is a critical enzyme in oxalate-dependent ATP synthesis and is the first Class III CoA-transferase for which a high resolution, three-dimensional structure has been determined (Ricagno, S., Jonsson, S., Richards, N., and Lindqvist, Y. (2003) EMBO J. 22, 3210-3219). We now report the first detailed kinetic characterizations of recombinant, wild type formyl-CoA transferase and a number of site-specific mutants, which suggest that catalysis proceeds via a series of anhydride intermediates. Further evidence for this mechanistic proposal is provided by the x-ray crystallographic observation of an acylenzyme intermediate that is formed when formyl-CoA transferase is incubated with oxalyl-CoA. The catalytic mechanism of formyl-CoA transferase is therefore established and is almost certainly employed by all other members of the Class III CoA-transferase family.
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{{ABSTRACT_PUBMED_15213226}}
==About this Structure==
==About this Structure==
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[[Category: Oxalate]]
[[Category: Oxalate]]
[[Category: Oxalate degradation]]
[[Category: Oxalate degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:31:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:07:16 2008''

Revision as of 03:07, 28 July 2008

Image:1vgr.png

Template:STRUCTURE 1vgr

Formyl-CoA transferase mutant Asp169 to Glu

Template:ABSTRACT PUBMED 15213226

About this Structure

1VGR is a Single protein structure of sequence from Oxalobacter formigenes. Full crystallographic information is available from OCA.

Reference

Kinetic and mechanistic characterization of the formyl-CoA transferase from Oxalobacter formigenes., Jonsson S, Ricagno S, Lindqvist Y, Richards NG, J Biol Chem. 2004 Aug 20;279(34):36003-12. Epub 2004 Jun 21. PMID:15213226

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