This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2hot

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2hot.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2hot.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hot| PDB=2hot | SCENE= }}
{{STRUCTURE_2hot| PDB=2hot | SCENE= }}
-
'''Phage selected homeodomain bound to modified DNA'''
+
===Phage selected homeodomain bound to modified DNA===
-
==Overview==
+
<!--
-
The homeodomain (HD)-DNA interface has been conserved over 500 million years of evolution. Despite this conservation, we have successfully re-engineered the engrailed HD to specifically recognize an unnatural nucleotide using a phage display selection. Here we report the synthesis of novel nucleosides and the selection of mutant HDs that bind these nucleotides using phage display. The high-resolution crystal structure of one mutant in complex with modified and unmodified DNA demonstrates that, even with the substantial perturbation to the interface, this selected mutant retains a canonical HD structure. Dissection of the contributions due to each of the selected mutations reveals that the majority of the modification-specific binding is accomplished by a single mutation (I47G) but that the remaining mutations retune the stability of the HD. These results afford a detailed look at a re-engineered protein-DNA interaction and provide insight into the opportunities for re-engineering highly conserved interfaces.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17240973}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17240973 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17240973}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Homeodomain]]
[[Category: Homeodomain]]
[[Category: Phage display]]
[[Category: Phage display]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:31:37 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:15:31 2008''

Revision as of 03:15, 28 July 2008

Image:2hot.png

Template:STRUCTURE 2hot

Phage selected homeodomain bound to modified DNA

Template:ABSTRACT PUBMED 17240973

About this Structure

2HOT is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Structure and properties of a re-engineered homeodomain protein-DNA interface., Simon MD, Feldman ME, Rauh D, Maris AE, Wemmer DE, Shokat KM, ACS Chem Biol. 2006 Dec 15;1(12):755-60. PMID:17240973

Page seeded by OCA on Mon Jul 28 06:15:31 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hot"
Personal tools