1w4u

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[[Image:1w4u.gif|left|200px]]
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{{Seed}}
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[[Image:1w4u.png|left|200px]]
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{{STRUCTURE_1w4u| PDB=1w4u | SCENE= }}
{{STRUCTURE_1w4u| PDB=1w4u | SCENE= }}
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'''NMR SOLUTION STRUCTURE OF THE UBIQUITIN CONJUGATING ENZYME UBCH5B'''
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===NMR SOLUTION STRUCTURE OF THE UBIQUITIN CONJUGATING ENZYME UBCH5B===
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==Overview==
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The ubiquitination pathway is the main pathway for protein degradation in eukaryotic cells. The attachment of ubiquitin to a substrate protein is catalyzed by three types of enzymes, namely a ubiquitin activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin ligase (E3). Here, the structure of the human ubiquitin-conjugating enzyme (E2) UbcH5B has been solved by a combination of homology modeling, NMR relaxation data and automated NOE assignments. Comparison to E2 structures solved previously by X-ray crystallography or NMR shows in all cases the same compact fold, but differences are observed in the orientation of both N and C-terminal alpha-helices. The N-terminal helix that is involved in binding to ubiquitin ligases (E3) displays a different position, which could have consequences for precise E2-E3 recognition. In addition, multiple conformations of the side-chain of Asn77 are found in solution, which contrasts the single hydrogen-bonded conformation in the crystal structures of E2 enzymes. The possible implication of this conformational freedom of Asn77 for its catalytic function is discussed.
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The line below this paragraph, {{ABSTRACT_PUBMED_15522302}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15522302 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15522302}}
==About this Structure==
==About this Structure==
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1W4U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W4U OCA].
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1W4U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W4U OCA].
==Reference==
==Reference==
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[[Category: Ligase]]
[[Category: Ligase]]
[[Category: Ubiquitination]]
[[Category: Ubiquitination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:09:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:18:35 2008''

Revision as of 03:18, 28 July 2008

Image:1w4u.png

Template:STRUCTURE 1w4u

NMR SOLUTION STRUCTURE OF THE UBIQUITIN CONJUGATING ENZYME UBCH5B

Template:ABSTRACT PUBMED 15522302

About this Structure

1W4U is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure of the ubiquitin-conjugating enzyme UbcH5B., Houben K, Dominguez C, van Schaik FM, Timmers HT, Bonvin AM, Boelens R, J Mol Biol. 2004 Nov 19;344(2):513-26. PMID:15522302

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