2fok

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{{STRUCTURE_2fok| PDB=2fok | SCENE= }}
{{STRUCTURE_2fok| PDB=2fok | SCENE= }}
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'''STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI'''
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===STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI===
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==Overview==
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FokI is a member an unusual class of restriction enzymes that recognize a specific DNA sequence and cleave nonspecifically a short distance away from that sequence. FokI consists of an N-terminal DNA recognition domain and a C-terminal cleavage domain. The bipartite nature of FokI has led to the development of artificial enzymes with novel specificities. We have solved the structure of FokI to 2.3 A resolution. The structure reveals a dimer, in which the dimerization interface is mediated by the cleavage domain. Each monomer has an overall conformation similar to that found in the FokI-DNA complex, with the cleavage domain packing alongside the DNA recognition domain. In corroboration with the cleavage data presented in the accompanying paper in this issue of Proceedings, we propose a model for FokI DNA cleavage that requires the dimerization of FokI on DNA to cleave both DNA strands.
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The line below this paragraph, {{ABSTRACT_PUBMED_9724743}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9724743 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9724743}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structure of FokI has implications for DNA cleavage., Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9724743 9724743]
Structure of FokI has implications for DNA cleavage., Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9724743 9724743]
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FokI dimerization is required for DNA cleavage., Bitinaite J, Wah DA, Aggarwal AK, Schildkraut I, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10570-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9724744 9724744]
[[Category: Planomicrobium okeanokoites]]
[[Category: Planomicrobium okeanokoites]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Nucleic acid recognition]]
[[Category: Nucleic acid recognition]]
[[Category: Type iis restriction endonuclease]]
[[Category: Type iis restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:08:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:23:10 2008''

Revision as of 03:23, 28 July 2008

Image:2fok.png

Template:STRUCTURE 2fok

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI

Template:ABSTRACT PUBMED 9724743

About this Structure

2FOK is a Single protein structure of sequence from Planomicrobium okeanokoites. Full crystallographic information is available from OCA.

Reference

Structure of FokI has implications for DNA cleavage., Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. PMID:9724743

FokI dimerization is required for DNA cleavage., Bitinaite J, Wah DA, Aggarwal AK, Schildkraut I, Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10570-5. PMID:9724744

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