1n6p

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{{STRUCTURE_1n6p| PDB=1n6p | SCENE= }}
{{STRUCTURE_1n6p| PDB=1n6p | SCENE= }}
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'''Crystal Structure of Human Rab5a A30E mutant complex with GppNHp'''
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===Crystal Structure of Human Rab5a A30E mutant complex with GppNHp===
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==Overview==
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GTPase domain crystal structures of Rab5a wild type and five variants with mutations in the phosphate-binding loop are reported here at resolutions up to 1.5 A. Of particular interest, the A30P mutant was crystallized in complexes with GDP, GDP+AlF(3), and authentic GTP, respectively. The other variant crystals were obtained in complexes with a non-hydrolyzable GTP analog, GppNHp. All structures were solved in the same crystal form, providing an unusual opportunity to compare structures of small GTPases with different catalytic rates. The A30P mutant exhibits dramatically reduced GTPase activity and forms a GTP-bound complex stable enough for crystallographic analysis. Importantly, the A30P structure with bound GDP plus AlF(3) has been solved in the absence of a GTPase-activating protein, and it may resemble that of a transition state intermediate. Conformational changes are observed between the GTP-bound form and the transition state intermediate, mainly in the switch II region containing the catalytic Gln(79) residue and independent of A30P mutation-induced local alterations in the P-loop. The structures suggest an important catalytic role for a P-loop backbone amide group, which is eliminated in the A30P mutant, and support the notion that the transition state of GTPase-mediated GTP hydrolysis is of considerable dissociative character.
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(as it appears on PubMed at http://www.pubmed.gov), where 12433916 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12433916}}
==About this Structure==
==About this Structure==
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[[Category: Gtpase]]
[[Category: Gtpase]]
[[Category: Rab]]
[[Category: Rab]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:09:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:24:57 2008''

Revision as of 03:24, 28 July 2008

Image:1n6p.png

Template:STRUCTURE 1n6p

Crystal Structure of Human Rab5a A30E mutant complex with GppNHp

Template:ABSTRACT PUBMED 12433916

About this Structure

1N6P is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

High resolution crystal structures of human Rab5a and five mutants with substitutions in the catalytically important phosphate-binding loop., Zhu G, Liu J, Terzyan S, Zhai P, Li G, Zhang XC, J Biol Chem. 2003 Jan 24;278(4):2452-60. Epub 2002 Nov 13. PMID:12433916

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