1zo3

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{{STRUCTURE_1zo3| PDB=1zo3 | SCENE= }}
{{STRUCTURE_1zo3| PDB=1zo3 | SCENE= }}
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'''The P-site and P/E-site tRNA structures fitted to P/I site codon.'''
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===The P-site and P/E-site tRNA structures fitted to P/I site codon.===
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==Overview==
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The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex.
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The line below this paragraph, {{ABSTRACT_PUBMED_15935757}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15935757 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15935757}}
==About this Structure==
==About this Structure==
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[[Category: Initiation of protein synthesis]]
[[Category: Initiation of protein synthesis]]
[[Category: Ribosome]]
[[Category: Ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:52:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:35:37 2008''

Revision as of 03:35, 28 July 2008

Image:1zo3.png

Template:STRUCTURE 1zo3

The P-site and P/E-site tRNA structures fitted to P/I site codon.

Template:ABSTRACT PUBMED 15935757

About this Structure

Full crystallographic information is available from OCA.

Reference

The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:15935757

Page seeded by OCA on Mon Jul 28 06:35:37 2008

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