2osl

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{{STRUCTURE_2osl| PDB=2osl | SCENE= }}
{{STRUCTURE_2osl| PDB=2osl | SCENE= }}
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'''Crystal structure of Rituximab Fab in complex with an epitope peptide'''
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===Crystal structure of Rituximab Fab in complex with an epitope peptide===
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==Overview==
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Rituximab is a widely used monoclonal antibody drug for treating certain lymphomas and autoimmune diseases. To understand the molecular mechanism of recognition of human CD20 by Rituximab, we determined the crystal structure of the Rituximab Fab in complex with a synthesized peptide comprising the CD20 epitope (residues 163-187) at 2.6-A resolution. The combining site of the Fab consists of four complementarity determining regions that form a large, deep pocket to accommodate the epitope peptide. The bound peptide assumes a unique cyclic conformation that is constrained by a disulfide bond and a rigid proline residue (Pro(172)). The (170)ANPS(173) motif of CD20 is deeply embedded into the pocket on the antibody surface and plays an essential role in the recognition and binding of Rituximab. The antigen-antibody interactions involve both hydrogen bonds and van der Waals contacts and display a high degree of structural and chemical complementarity. These results provide a molecular basis for the specific recognition of CD20 by Rituximab as well as valuable information for development of improved antibody drugs with better specificity and higher affinity.
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(as it appears on PubMed at http://www.pubmed.gov), where 17395584 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17395584}}
==About this Structure==
==About this Structure==
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[[Category: Fab-peptide complex]]
[[Category: Fab-peptide complex]]
[[Category: Rituximab]]
[[Category: Rituximab]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:34:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:53:41 2008''

Revision as of 03:53, 28 July 2008

Image:2osl.png


PDB ID 2osl

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2osl, resolution 2.60Å ()
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Crystal structure of Rituximab Fab in complex with an epitope peptide

Template:ABSTRACT PUBMED 17395584

About this Structure

2OSL is a Single protein structure of sequence from Homo sapiens, mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for recognition of CD20 by therapeutic antibody Rituximab., Du J, Wang H, Zhong C, Peng B, Zhang M, Li B, Huo S, Guo Y, Ding J, J Biol Chem. 2007 May 18;282(20):15073-80. Epub 2007 Mar 29. PMID:17395584

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