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1nku

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[[Image:1nku.gif|left|200px]]
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{{STRUCTURE_1nku| PDB=1nku | SCENE= }}
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'''NMR Solution Structure of Zinc-binding protein 3-methyladenine DNA glycosylase I (TAG)'''
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===NMR Solution Structure of Zinc-binding protein 3-methyladenine DNA glycosylase I (TAG)===
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==Overview==
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The Escherichia coli 3-methyladenine DNA glycosylase I (TAG) is a DNA repair enzyme that excises 3-methyladenine in DNA and is the smallest member of the helix-hairpin-helix (HhH) superfamily of DNA glycosylases. Despite many studies over the last 25 years, there has been no suggestion that TAG was a metalloprotein. However, here we establish by heteronuclear NMR and other spectroscopic methods that TAG binds 1 eq of Zn2+ extremely tightly. A family of refined NMR structures shows that 4 conserved residues contributed from the amino- and carboxyl-terminal regions of TAG (Cys4, His17, His175, and Cys179) form a Zn2+ binding site. The Zn2+ ion serves to tether the otherwise unstructured amino- and carboxyl-terminal regions of TAG. We propose that this unexpected "zinc snap" motif in the TAG family (CX(12-17)HX(approximately 150)HX(3)C) serves to stabilize the HhH domain thereby mimicking the functional role of protein-protein interactions in larger HhH superfamily members.
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{{ABSTRACT_PUBMED_12654914}}
==About this Structure==
==About this Structure==
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1NKU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKU OCA].
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1NKU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKU OCA].
==Reference==
==Reference==
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[[Category: Stivers, J T.]]
[[Category: Stivers, J T.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:39:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:58:21 2008''

Revision as of 03:58, 28 July 2008

Image:1nku.png

Template:STRUCTURE 1nku

NMR Solution Structure of Zinc-binding protein 3-methyladenine DNA glycosylase I (TAG)

Template:ABSTRACT PUBMED 12654914

About this Structure

1NKU is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

A novel zinc snap motif conveys structural stability to 3-methyladenine DNA glycosylase I., Kwon K, Cao C, Stivers JT, J Biol Chem. 2003 May 23;278(21):19442-6. Epub 2003 Mar 24. PMID:12654914

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