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1pyv

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[[Image:1pyv.gif|left|200px]]
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{{STRUCTURE_1pyv| PDB=1pyv | SCENE= }}
{{STRUCTURE_1pyv| PDB=1pyv | SCENE= }}
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'''NMR solution structure of the mitochondrial F1b presequence peptide from Nicotiana plumbaginifolia'''
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===NMR solution structure of the mitochondrial F1b presequence peptide from Nicotiana plumbaginifolia===
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==Overview==
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We have isolated, characterized and determined the three-dimensional NMR solution structure of the presequence of ATPsynthase F1beta subunit from Nicotiana plumbaginifolia. A general method for purification of presequences is presented. The method is based on overexpression of a mutant precursor containing a methionine residue introduced at the processing site, followed by CNBr-cleavage and purification of the presequence on a cation-exchange column. The F1beta presequence, 53 amino acid residues long, retained its native properties as evidenced by inhibition of in vitro mitochondrial import and processing at micromolar concentrations. CD spectroscopy revealed that the F1beta presequence formed an alpha-helical structure in membrane mimetic environments such as SDS and DPC micelles (approximately 50% alpha-helix), and in acidic phospholipid bicelles (approximately 60% alpha-helix). The NMR solution structure of the F1beta presequence in SDS micelles was determined on the basis of 518 distance and 21 torsion angle constraints. The structure was found to contain two helices, an N-terminal amphipathic alpha-helix (residues 4-15) and a C-terminal alpha-helix (residues 43-53), separated by a largely unstructured 27 residue long internal domain. The N-terminal amphipathic alpha-helix forms the putative Tom20 receptor binding site, whereas the C-terminal alpha-helix is located upstream of the mitochondrial processing peptidase cleavage site.
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{{ABSTRACT_PUBMED_15037074}}
==About this Structure==
==About this Structure==
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1PYV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_plumbaginifolia Nicotiana plumbaginifolia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PYV OCA].
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1PYV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_plumbaginifolia Nicotiana plumbaginifolia]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PYV OCA].
==Reference==
==Reference==
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[[Category: Stahl, A.]]
[[Category: Stahl, A.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:39:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:59:18 2008''

Revision as of 03:59, 28 July 2008

Image:1pyv.png

Template:STRUCTURE 1pyv

NMR solution structure of the mitochondrial F1b presequence peptide from Nicotiana plumbaginifolia

Template:ABSTRACT PUBMED 15037074

About this Structure

1PYV is a Single protein structure of sequence from Nicotiana plumbaginifolia. Full experimental information is available from OCA.

Reference

NMR solution structure of the mitochondrial F1beta presequence from Nicotiana plumbaginifolia., Moberg P, Nilsson S, Stahl A, Eriksson AC, Glaser E, Maler L, J Mol Biol. 2004 Mar 5;336(5):1129-40. PMID:15037074

Page seeded by OCA on Mon Jul 28 06:59:18 2008

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