1p7i

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{{STRUCTURE_1p7i| PDB=1p7i | SCENE= }}
{{STRUCTURE_1p7i| PDB=1p7i | SCENE= }}
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'''CRYSTAL STRUCTURE OF ENGRAILED HOMEODOMAIN MUTANT K52A'''
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===CRYSTAL STRUCTURE OF ENGRAILED HOMEODOMAIN MUTANT K52A===
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==Overview==
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We report the crystal structures and biophysical characterization of two stabilized mutants of the Drosophila Engrailed homeodomain that have been engineered to minimize electrostatic repulsion. Four independent copies of each mutant occupy the crystal lattice, and comparison of these structures illustrates variation that can be partly ascribed to networks of correlated conformational adjustments. Central to one network is leucine 26 (Leu26), which occupies alternatively two side chain rotameric conformations (-gauche and trans) and different positions within the hydrophobic core. Similar sets of conformational substates are observed in other Engrailed structures and in another homeodomain. The pattern of structural adjustments can account for NMR relaxation data and sequence co-variation networks in the wider homeodomain family. It may also explain the dysfunction associated with a P26L mutation in the human ARX homeodomain protein. Finally, we observe a novel dipolar interaction between a conserved tryptophan and a water molecule positioned along the normal to the indole ring. This interaction may explain the distinctive fluorescent properties of the homeodomain family.
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{{ABSTRACT_PUBMED_12923178}}
==About this Structure==
==About this Structure==
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[[Category: Stollar, E J.]]
[[Category: Stollar, E J.]]
[[Category: Dna binding protein]]
[[Category: Dna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:02:39 2008''

Revision as of 04:02, 28 July 2008

Image:1p7i.png

Template:STRUCTURE 1p7i

CRYSTAL STRUCTURE OF ENGRAILED HOMEODOMAIN MUTANT K52A

Template:ABSTRACT PUBMED 12923178

About this Structure

1P7I is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Crystal structures of engrailed homeodomain mutants: implications for stability and dynamics., Stollar EJ, Mayor U, Lovell SC, Federici L, Freund SM, Fersht AR, Luisi BF, J Biol Chem. 2003 Oct 31;278(44):43699-708. Epub 2003 Aug 15. PMID:12923178

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