2jo9

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[[Image:2jo9.gif|left|200px]]
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{{STRUCTURE_2jo9| PDB=2jo9 | SCENE= }}
{{STRUCTURE_2jo9| PDB=2jo9 | SCENE= }}
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'''Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED'''
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===Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED===
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==Overview==
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In this work, we study the role of phosphorylation as a regulatory mechanism for the interaction between the E3 ubiquitin ligase ItchWW3 domain and two PPxY motifs of one of its targets, the Epstein-Barr virus latent membrane protein 2A. Whereas ligand phosphorylation only diminishes binding, domain phosphorylation at residue T30 abrogates it. We show that two ItchWW domains can be phosphorylated at this position, using CK2 and PKA kinases and/or with stimulated T lymphocyte lysates. To better understand the regulation process, we determined the NMR structures of the ItchWW3-PPxY complex and of the phosphoT30-ItchWW3 variant. The peptide binds the domain using both XP and tyrosine grooves. A hydrogen bond from T30 to the ligand is also detected. This hydrogen-bond formation is precluded in the variant, explaining the inhibition upon phosphorylation. Our results suggest that phosphorylation at position 30 in ItchWW domains can be a mechanism to inhibit target recognition in vivo.
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(as it appears on PubMed at http://www.pubmed.gov), where 17437719 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17437719}}
==About this Structure==
==About this Structure==
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2JO9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JO9 OCA].
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2JO9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JO9 OCA].
==Reference==
==Reference==
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[[Category: Lmp2a]]
[[Category: Lmp2a]]
[[Category: Ww]]
[[Category: Ww]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:06:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:05:55 2008''

Revision as of 04:06, 28 July 2008

Image:2jo9.png

Template:STRUCTURE 2jo9

Mouse Itch 3rd WW domain complex with the Epstein-Barr virus latent membrane protein 2A derived peptide EEPPPPYED

Template:ABSTRACT PUBMED 17437719

About this Structure

2JO9 is a Protein complex structure of sequences from Mus musculus. Full experimental information is available from OCA.

Reference

NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands., Morales B, Ramirez-Espain X, Shaw AZ, Martin-Malpartida P, Yraola F, Sanchez-Tillo E, Farrera C, Celada A, Royo M, Macias MJ, Structure. 2007 Apr;15(4):473-83. PMID:17437719

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