1uef

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{{STRUCTURE_1uef| PDB=1uef | SCENE= }}
{{STRUCTURE_1uef| PDB=1uef | SCENE= }}
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'''Crystal Structure of Dok1 PTB Domain Complex'''
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===Crystal Structure of Dok1 PTB Domain Complex===
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==Overview==
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Dok1 is a common substrate of activated protein-tyrosine kinases. It is rapidly tyrosine-phosphorylated in response to receptor tyrosine activation and interacts with ras GTPase-activating protein and Nck, leading to inhibition of ras signaling pathway activation and the c-Jun N-terminal kinase (JNK) and c-Jun activation, respectively. In chronic myelogenous leukemia cells, it has shown constitutive phosphorylation. The N-terminal phosphotyrosine binding (PTB) domain of Dok1 can recognize and bind specifically to phosphotyrosine-containing motifs of receptors. Here we report the crystal structure of the Dok1 PTB domain alone and in complex with a phosphopeptide derived from RET receptor tyrosine kinase. The structure consists of a beta-sandwich composed of two nearly orthogonal, 7-stranded, antiparallel beta-sheets, and it is capped at one side by a C-terminal alpha-helix. The RET phosphopeptide binds to Dok1 via a surface groove formed between strand beta5 and the C-terminal alpha-helix of the PTB domain. The structures reveal the molecular basis for the specific recognition of RET by the Dok1 PTB domain. We also show that Dok1 does not recognize peptide sequences from TrkA and IL-4, which are recognized by Shc and IRS1, respectively.
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(as it appears on PubMed at http://www.pubmed.gov), where 14607833 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14607833}}
==About this Structure==
==About this Structure==
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[[Category: Protein binding]]
[[Category: Protein binding]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:06:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:06:24 2008''

Revision as of 04:06, 28 July 2008

Image:1uef.png

Template:STRUCTURE 1uef

Crystal Structure of Dok1 PTB Domain Complex

Template:ABSTRACT PUBMED 14607833

About this Structure

1UEF is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for the specific recognition of RET by the Dok1 phosphotyrosine binding domain., Shi N, Ye S, Bartlam M, Yang M, Wu J, Liu Y, Sun F, Han X, Peng X, Qiang B, Yuan J, Rao Z, J Biol Chem. 2004 Feb 6;279(6):4962-9. Epub 2003 Nov 7. PMID:14607833

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