2f7t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2f7t.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2f7t.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2f7t| PDB=2f7t | SCENE= }}
{{STRUCTURE_2f7t| PDB=2f7t | SCENE= }}
-
'''Crystal structure of the catalytic domain of Mos1 mariner transposase'''
+
===Crystal structure of the catalytic domain of Mos1 mariner transposase===
-
==Overview==
+
<!--
-
We present the crystal structure of the catalytic domain of Mos1 transposase, a member of the Tc1/mariner family of transposases. The structure comprises an RNase H-like core, bringing together an aspartic acid triad to form the active site, capped by N- and C-terminal alpha-helices. We have solved structures with either one Mg2+ or two Mn2+ ions in the active site, consistent with a two-metal mechanism for catalysis. The lack of hairpin-stabilizing structural motifs is consistent with the absence of a hairpin intermediate in Mos1 excision. We have built a model for the DNA-binding domain of Mos1 transposase, based on the structure of the bipartite DNA-binding domain of Tc3 transposase. Combining this with the crystal structure of the catalytic domain provides a model for the paired-end complex formed between a dimer of Mos1 transposase and inverted repeat DNA. The implications for the mechanisms of first and second strand cleavage are discussed.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16511570}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16511570 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16511570}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Ddd motif]]
[[Category: Ddd motif]]
[[Category: Rnase-h like fold]]
[[Category: Rnase-h like fold]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:33:51 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:11:58 2008''

Revision as of 04:11, 28 July 2008

Image:2f7t.png

Template:STRUCTURE 2f7t

Crystal structure of the catalytic domain of Mos1 mariner transposase

Template:ABSTRACT PUBMED 16511570

About this Structure

2F7T is a Single protein structure of sequence from Drosophila mauritiana. Full crystallographic information is available from OCA.

Reference

Mechanism of Mos1 transposition: insights from structural analysis., Richardson JM, Dawson A, O'Hagan N, Taylor P, Finnegan DJ, Walkinshaw MD, EMBO J. 2006 Mar 22;25(6):1324-34. Epub 2006 Mar 2. PMID:16511570

Page seeded by OCA on Mon Jul 28 07:11:58 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2f7t"
Personal tools