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3b5u

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{{STRUCTURE_3b5u| PDB=3b5u | SCENE= }}
{{STRUCTURE_3b5u| PDB=3b5u | SCENE= }}
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'''Actin filament model from extended form of acromsomal bundle in the Limulus sperm'''
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===Actin filament model from extended form of acromsomal bundle in the Limulus sperm===
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==Overview==
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Actin carries out many of its cellular functions through its filamentous form; thus, understanding the detailed structure of actin filaments is an essential step in achieving a mechanistic understanding of actin function. The acrosomal bundle in the Limulus sperm has been shown to be a quasi-crystalline array with an asymmetric unit composed of a filament with 14 actin-scruin pairs. The bundle in its true discharge state penetrates the jelly coat of the egg. Our previous electron crystallographic reconstruction demonstrated that the actin filament cross-linked by scruin in this acrosomal bundle state deviates significantly from a perfect F-actin helix. In that study, the tertiary structure of each of the 14 actin protomers in the asymmetric unit of the bundle filament was assumed to be constant. In the current study, an actin filament atomic model in the acrosomal bundle has been refined by combining rigid-body docking with multiple actin crystal structures from the Protein Data Bank and constrained energy minimization. Our observation demonstrates that actin protomers adopt different tertiary conformations when they form an actin filament in the bundle. The scruin and bundle packing forces appear to influence the tertiary and quaternary conformations of actin in the filament of this biologically active bundle.
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{{ABSTRACT_PUBMED_18022194}}
==About this Structure==
==About this Structure==
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[[Category: Cryoem]]
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Revision as of 04:37, 28 July 2008

Image:3b5u.png

Template:STRUCTURE 3b5u

Actin filament model from extended form of acromsomal bundle in the Limulus sperm

Template:ABSTRACT PUBMED 18022194

About this Structure

3B5U is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

Crystallographic conformers of actin in a biologically active bundle of filaments., Cong Y, Topf M, Sali A, Matsudaira P, Dougherty M, Chiu W, Schmid MF, J Mol Biol. 2008 Jan 11;375(2):331-6. Epub 2007 Oct 16. PMID:18022194

Page seeded by OCA on Mon Jul 28 07:37:09 2008

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