2f8p

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{{STRUCTURE_2f8p| PDB=2f8p | SCENE= }}
{{STRUCTURE_2f8p| PDB=2f8p | SCENE= }}
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'''Crystal structure of obelin following Ca2+ triggered bioluminescence suggests neutral coelenteramide as the primary excited state'''
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===Crystal structure of obelin following Ca2+ triggered bioluminescence suggests neutral coelenteramide as the primary excited state===
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==Overview==
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The crystal structure at 1.93-A resolution is determined for the Ca2+-discharged obelin containing three bound calcium ions as well as the product of the bioluminescence reaction, coelenteramide. This finding extends the series of available spatial structures of the ligand-dependent conformations of the protein to four, the obelin itself, and those after the bioluminescence reaction with or without bound Ca2+ and/or coelenteramide. Among these structures, global conformational changes are small, typical of the class of "calcium signal modulators" within the EF-hand protein superfamily. Nevertheless, in the active site there are significant repositions of two residues. The His-175 imidazole ring flips becoming almost perpendicular to the original orientation corroborating the crucial importance of this residue for triggering bioluminescence. Tyr-138 hydrogen bonded to the coelenterazine N1-atom in unreacted obelin is moved away from the binding cavity after reaction. However, this Tyr is displaced by a water molecule from within the cavity, which now forms a hydrogen bond to the same atom, the amide N of coelenteramide. From this observation, a reaction scheme is proposed that would result in the neutral coelenteramide as the primary excited state product in photoprotein bioluminescence. From such a higher energy state it is now energetically feasible to account for the shorter wavelength bioluminescence spectra obtained from some photoprotein mutants or to populate the lower energy state of the phenolate anion to yield the blue bioluminescence ordinarily observed from native photoproteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 16467137 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16467137}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state., Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2570-5. Epub 2006 Feb 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16467137 16467137]
Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state., Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2570-5. Epub 2006 Feb 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16467137 16467137]
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Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure., Liu ZJ, Vysotski ES, Chen CJ, Rose JP, Lee J, Wang BC, Protein Sci. 2000 Nov;9(11):2085-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11152120 11152120]
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Structural basis for the emission of violet bioluminescence from a W92F obelin mutant., Deng L, Vysotski ES, Liu ZJ, Markova SV, Malikova NP, Lee J, Rose J, Wang BC, FEBS Lett. 2001 Oct 12;506(3):281-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11602262 11602262]
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Violet bioluminescence and fast kinetics from W92F obelin: structure-based proposals for the bioluminescence triggering and the identification of the emitting species., Vysotski ES, Liu ZJ, Markova SV, Blinks JR, Deng L, Frank LA, Herko M, Malikova NP, Rose JP, Wang BC, Lee J, Biochemistry. 2003 May 27;42(20):6013-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12755603 12755603]
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Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine., Liu ZJ, Vysotski ES, Deng L, Lee J, Rose J, Wang BC, Biochem Biophys Res Commun. 2003 Nov 14;311(2):433-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14592432 14592432]
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Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence., Deng L, Markova SV, Vysotski ES, Liu ZJ, Lee J, Rose J, Wang BC, J Biol Chem. 2004 Aug 6;279(32):33647-52. Epub 2004 May 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15155735 15155735]
[[Category: Obelia longissima]]
[[Category: Obelia longissima]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Southeast collaboratory for structural genomic]]
[[Category: Southeast collaboratory for structural genomic]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:35:51 2008''
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Revision as of 04:50, 28 July 2008

Image:2f8p.png

Template:STRUCTURE 2f8p

Crystal structure of obelin following Ca2+ triggered bioluminescence suggests neutral coelenteramide as the primary excited state

Template:ABSTRACT PUBMED 16467137

About this Structure

2F8P is a Single protein structure of sequence from Obelia longissima. Full crystallographic information is available from OCA.

Reference

Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state., Liu ZJ, Stepanyuk GA, Vysotski ES, Lee J, Markova SV, Malikova NP, Wang BC, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2570-5. Epub 2006 Feb 8. PMID:16467137

Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure., Liu ZJ, Vysotski ES, Chen CJ, Rose JP, Lee J, Wang BC, Protein Sci. 2000 Nov;9(11):2085-93. PMID:11152120

Structural basis for the emission of violet bioluminescence from a W92F obelin mutant., Deng L, Vysotski ES, Liu ZJ, Markova SV, Malikova NP, Lee J, Rose J, Wang BC, FEBS Lett. 2001 Oct 12;506(3):281-5. PMID:11602262

Violet bioluminescence and fast kinetics from W92F obelin: structure-based proposals for the bioluminescence triggering and the identification of the emitting species., Vysotski ES, Liu ZJ, Markova SV, Blinks JR, Deng L, Frank LA, Herko M, Malikova NP, Rose JP, Wang BC, Lee J, Biochemistry. 2003 May 27;42(20):6013-24. PMID:12755603

Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine., Liu ZJ, Vysotski ES, Deng L, Lee J, Rose J, Wang BC, Biochem Biophys Res Commun. 2003 Nov 14;311(2):433-9. PMID:14592432

Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence., Deng L, Markova SV, Vysotski ES, Liu ZJ, Lee J, Rose J, Wang BC, J Biol Chem. 2004 Aug 6;279(32):33647-52. Epub 2004 May 20. PMID:15155735

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