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| - | [[Image:2a01.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2a01| PDB=2a01 | SCENE= }} | | {{STRUCTURE_2a01| PDB=2a01 | SCENE= }} |
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| - | '''Crystal Structure of Lipid-free Human Apolipoprotein A-I'''
| + | ===Crystal Structure of Lipid-free Human Apolipoprotein A-I=== |
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| - | ==Overview==
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| - | Despite three decades of extensive studies on human apolipoprotein A-I (apoA-I), the major protein component in high-density lipoproteins, the molecular basis for its antiatherogenic function is elusive, in part because of lack of a structure of the full-length protein. We describe here the crystal structure of lipid-free apoA-I at 2.4 A. The structure shows that apoA-I is comprised of an N-terminal four-helix bundle and two C-terminal helices. The N-terminal domain plays a prominent role in maintaining its lipid-free conformation, indicating that mutants with truncations in this region form inadequate models for explaining functional properties of apoA-I. A model for transformation of the lipid-free conformation to the high-density lipoprotein-bound form follows from an analysis of solvent-accessible hydrophobic patches on the surface of the structure and their proximity to the hydrophobic core of the four-helix bundle. The crystal structure of human apoA-I displays a hitherto-unobserved array of positively and negatively charged areas on the surface. Positioning of the charged surface patches relative to hydrophobic regions near the C terminus of the protein offers insights into its interaction with cell-surface components of the reverse cholesterol transport pathway and antiatherogenic properties of this protein. This structure provides a much-needed structural template for exploration of molecular mechanisms by which human apoA-I ameliorates atherosclerosis and inflammatory diseases.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16452169}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16452169 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16452169}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Murthy, K H.M.]] | | [[Category: Murthy, K H.M.]] |
| | [[Category: Four-helix bundle]] | | [[Category: Four-helix bundle]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:26:25 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:51:53 2008'' |
Revision as of 04:51, 28 July 2008
Template:STRUCTURE 2a01
Crystal Structure of Lipid-free Human Apolipoprotein A-I
Template:ABSTRACT PUBMED 16452169
About this Structure
2A01 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human apolipoprotein A-I: insights into its protective effect against cardiovascular diseases., Ajees AA, Anantharamaiah GM, Mishra VK, Hussain MM, Murthy HM, Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2126-31. Epub 2006 Feb 1. PMID:16452169
Page seeded by OCA on Mon Jul 28 07:51:53 2008
