2a7q

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{{STRUCTURE_2a7q| PDB=2a7q | SCENE= }}
{{STRUCTURE_2a7q| PDB=2a7q | SCENE= }}
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'''Crystal structure of human dCK complexed with clofarabine and ADP'''
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===Crystal structure of human dCK complexed with clofarabine and ADP===
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==Overview==
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Clofarabine [2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-9H-purin-6-amine] is a hybrid of the widely used anticancer drugs cladribine and fludarabine. It is the precursor of an effective chemotherapeutic agent for leukemias and other hematological malignancies and received accelerated approval by the FDA for the treatment of pediatric patients with relapsed or refractory acute lymphoblastic leukemia. Clofarabine is phosphorylated intracellularly by human deoxycytidine kinase (dCK) to the 5'-monophosphate, which is the rate-limiting step in activation of the prodrug. dCK has a broad substrate specificity, with a much higher activity to deoxycytidine than to deoxyadenosine and deoxyguanosine. As a purine-nucleoside analog, clofarabine is a better substrate of dCK than deoxycytidine. The crystal structure of dCK has been solved previously in complex with pyrimidine nucleosides and ADP [Sabini et al. (2003), Nature Struct. Biol. 10, 513-519]. In the current study, the crystal structure of clofarabine- and ADP-bound dCK was solved to 2.55 angstroms by molecular replacement. It appears that the enzyme takes the same conformation as in the structures of the pyrimidine nucleoside-bound complexes. The interactions between 2-Cl and its surrounding hydrophobic residues contribute to the high catalytic efficiency of dCK for clofarabine.
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(as it appears on PubMed at http://www.pubmed.gov), where 16421443 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16421443}}
==About this Structure==
==About this Structure==
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[[Category: Zhang, Y.]]
[[Category: Zhang, Y.]]
[[Category: Alpha/beta parallel beta-sheet of 5 strand]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:42:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:56:46 2008''

Revision as of 04:56, 28 July 2008

Image:2a7q.png

Template:STRUCTURE 2a7q

Crystal structure of human dCK complexed with clofarabine and ADP

Template:ABSTRACT PUBMED 16421443

About this Structure

2A7Q is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structure of human deoxycytidine kinase in complex with clofarabine reveals key interactions for prodrug activation., Zhang Y, Secrist JA 3rd, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):133-9. Epub 2006, Jan 18. PMID:16421443

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