2jn3

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{{STRUCTURE_2jn3| PDB=2jn3 | SCENE= }}
{{STRUCTURE_2jn3| PDB=2jn3 | SCENE= }}
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'''NMR structure of cl-BABP complexed to chenodeoxycholic acid'''
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===NMR structure of cl-BABP complexed to chenodeoxycholic acid===
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==Overview==
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Bile acids are physiological detergents facilitating absorption, transport, and distribution of lipid-soluble vitamins and dietary fats;they also play a role as signaling molecules that activate nuclear receptors and regulate cholesterol metabolism. Bile acid circulation is mediated by bile acid binding proteins (BABPs), and a detailed structural study of the complex of BABPs with bile salts has become a key issue for the complete understanding of the role of these proteins and their involvement in cholesterol homeostasis. The solution structure here reported describes, at variance with previously determined singly ligated structures, a BABP in a ternary complex with two bile acid molecules, obtained by employing a variety of NMR experiments. Exchange processes between the two bound chenodeoxycholate molecules as well as the more superficial ligand and the free pool have been detected through ROESY and diffusion experiments. Significant backbone flexibility has been observed in regions located at the protein open end, facilitating bile salts exchange. A detailed description of the protonation states and tautomeric forms of histidines strongly supports the view that histidine protonation modulates backbone flexibility and regulates ligand binding. This structure opens the way to targeted site-directed mutagenesis and interaction studies to investigate both binding and nuclear localization mechanisms.
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{{ABSTRACT_PUBMED_17929837}}
==About this Structure==
==About this Structure==
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2JN3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JN3 OCA].
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2JN3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JN3 OCA].
==Reference==
==Reference==
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[[Category: Lipid binding protein]]
[[Category: Lipid binding protein]]
[[Category: Nmr]]
[[Category: Nmr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:28:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:00:39 2008''

Revision as of 05:00, 28 July 2008

Image:2jn3.png

Template:STRUCTURE 2jn3

NMR structure of cl-BABP complexed to chenodeoxycholic acid

Template:ABSTRACT PUBMED 17929837

About this Structure

2JN3 is a Single protein structure of sequence from Gallus gallus. Full experimental information is available from OCA.

Reference

Structural and dynamic determinants of ligand binding in the ternary complex of chicken liver bile acid binding protein with two bile salts revealed by NMR., Eliseo T, Ragona L, Catalano M, Assfalg M, Paci M, Zetta L, Molinari H, Cicero DO, Biochemistry. 2007 Nov 6;46(44):12557-67. Epub 2007 Oct 11. PMID:17929837

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