1prz

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{{STRUCTURE_1prz| PDB=1prz | SCENE= }}
{{STRUCTURE_1prz| PDB=1prz | SCENE= }}
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'''Crystal structure of pseudouridine synthase RluD catalytic module'''
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===Crystal structure of pseudouridine synthase RluD catalytic module===
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==Overview==
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Pseudouridine (5-beta-D-ribofuranosyluracil, Psi) is the most commonly found modified base in RNA. Conversion of uridine to Psi is performed enzymatically in both prokaryotes and eukaryotes by pseudouridine synthases (EC 4.2.1.70). The Escherichia coli Psi-synthase RluD modifies uridine to Psi at positions 1911, 1915 and 1917 within 23S rRNA. RluD also possesses a second function related to proper assembly of the 50S ribosomal subunit that is independent of Psi-synthesis. Here, we report the crystal structure of the catalytic module of RluD (residues 68-326; DeltaRluD) refined at 1.8A to a final R-factor of 21.8% (R(free)=24.3%). DeltaRluD is a monomeric enzyme having an overall mixed alpha/beta fold. The DeltaRluD molecule consists of two subdomains, a catalytic subdomain and C-terminal subdomain with the RNA-binding cleft formed by loops extending from the catalytic sub-domain. The catalytic sub-domain of DeltaRluD has a similar fold as in TruA, TruB and RsuA, with the location of the RNA-binding cleft, active-site and conserved, catalytic Asp residue superposing in all four structures. Superposition of the crystal structure of TruB bound to a T-stem loop with RluD reveals that similar RNA-protein interactions for the flipped-out uridine base would exist in both structures, implying that base-flipping is necessary for catalysis. This observation also implies that the specificity determinants for site-specific RNA-binding and recognition likely reside in parts of RluD beyond the active site.
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{{ABSTRACT_PUBMED_14659742}}
==About this Structure==
==About this Structure==
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[[Category: Rlud]]
[[Category: Rlud]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:11:20 2008''

Revision as of 05:11, 28 July 2008

Image:1prz.png

Template:STRUCTURE 1prz

Crystal structure of pseudouridine synthase RluD catalytic module

Template:ABSTRACT PUBMED 14659742

About this Structure

1PRZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of the RluD pseudouridine synthase catalytic module, an enzyme that modifies 23S rRNA and is essential for normal cell growth of Escherichia coli., Sivaraman J, Iannuzzi P, Cygler M, Matte A, J Mol Biol. 2004 Jan 2;335(1):87-101. PMID:14659742

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