2oe8

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{{STRUCTURE_2oe8| PDB=2oe8 | SCENE= }}
{{STRUCTURE_2oe8| PDB=2oe8 | SCENE= }}
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'''1.8 A X-ray crystal structure of Apramycin complex with RNA fragment GGGCGUCGCUAGUACC/CGGUACUAAAAGUCGCC containing the human ribosomal decoding A site: RNA construct with 5'-overhang'''
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===1.8 A X-ray crystal structure of Apramycin complex with RNA fragment GGGCGUCGCUAGUACC/CGGUACUAAAAGUCGCC containing the human ribosomal decoding A site: RNA construct with 5'-overhang===
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==Overview==
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Aminoglycoside antibiotics bind specifically to the bacterial ribosomal decoding-site RNA and thereby interfere with fidelity but not efficiency of translation. Apramycin stands out among aminoglycosides for its mechanism of action which is based on blocking translocation and its ability to bind also to the eukaryotic decoding site despite differences in key residues required for apramycin recognition by the bacterial target. To elucidate molecular recognition of the eukaryotic decoding site by apramycin we have determined the crystal structure of an oligoribonucleotide containing the human sequence free and in complex with the antibiotic at 1.5 A resolution. The drug binds in the deep groove of the RNA which forms a continuously stacked helix comprising non-canonical C.A and G.A base pairs and a bulged-out adenine. The binding mode of apramycin at the human decoding-site RNA is distinct from aminoglycoside recognition of the bacterial target, suggesting a molecular basis for the actions of apramycin in eukaryotes and bacteria.
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(as it appears on PubMed at http://www.pubmed.gov), where 17258916 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17258916}}
==About this Structure==
==About this Structure==
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[[Category: Ribosomal decoding site]]
[[Category: Ribosomal decoding site]]
[[Category: Rna duplex]]
[[Category: Rna duplex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:43:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:12:36 2008''

Revision as of 05:12, 28 July 2008

Image:2oe8.png

Template:STRUCTURE 2oe8

1.8 A X-ray crystal structure of Apramycin complex with RNA fragment GGGCGUCGCUAGUACC/CGGUACUAAAAGUCGCC containing the human ribosomal decoding A site: RNA construct with 5'-overhang

Template:ABSTRACT PUBMED 17258916

About this Structure

Full crystallographic information is available from OCA.

Reference

Apramycin recognition by the human ribosomal decoding site., Hermann T, Tereshko V, Skripkin E, Patel DJ, Blood Cells Mol Dis. 2007 May-Jun;38(3):193-8. Epub 2007 Jan 29. PMID:17258916

Page seeded by OCA on Mon Jul 28 08:12:36 2008

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