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| | {{STRUCTURE_1r3o| PDB=1r3o | SCENE= }} | | {{STRUCTURE_1r3o| PDB=1r3o | SCENE= }} |
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| - | '''Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution'''
| + | ===Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution=== |
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| - | ==Overview==
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| - | Nucleic acid molecules in the mirror image or L-configuration are unknown in nature and are extraordinarily resistant to biological degradation. The identification of functional L-oligonucleotides called Spiegelmers offers a novel approach for drug discovery based on RNA. The sequence r(CUGGGCGG).r(CCGCCUGG) was chosen as a model system for structural analysis of helices in the L-configuration as the structure of the D-form of this sequence has previously been determined in structural studies of 5S RNA domains, in particular domain E of the Thermus flavus 5S rRNA [Perbandt et al. (2001), Acta Cryst. D57, 219-224]. Unexpectedly, the results of crystallization trials showed little similarity between the D- and the L-forms of the duplex in either the crystallization hits or the diffraction performance. The crystal structure of this L-RNA duplex has been determined at 1.9 A resolution with R(work) and R(free) of 23.8 and 28.6%, respectively. The crystals belong to space group R32, with unit-cell parameters a = 45.7, c = 264.6 A. Although there are two molecules in the asymmetric unit rather than one, the structure of the L-form arranges helical pairs in a head-to-tail fashion to form pseudo-continuous infinite helices in the crystal as in the D-form. On the other hand, the wobble-like G.C(+) base pair seen in the D-RNA analogue does not appear in the L-RNA duplex, which forms a regular double-helical structure with typical Watson-Crick base pairing.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_14684885}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 14684885 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_14684885}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Vallazza, M.]] | | [[Category: Vallazza, M.]] |
| | [[Category: L-rna]] | | [[Category: L-rna]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:02:09 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:15:52 2008'' |
Revision as of 05:15, 28 July 2008
Template:STRUCTURE 1r3o
Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution
Template:ABSTRACT PUBMED 14684885
About this Structure
Full crystallographic information is available from OCA.
Reference
First look at RNA in L-configuration., Vallazza M, Perbandt M, Klussmann S, Rypniewski W, Einspahr HM, Erdmann VA, Betzel Ch, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):1-7. Epub 2003 Dec, 18. PMID:14684885
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