1m1f

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Revision as of 18:56, 20 November 2007

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spinqualitylabelsall models 1m1f, resolution 1.4Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Kid toxin protein from E.coli plasmid R1

Overview

We have determined the structure of Kid toxin protein from E. coli plasmid, R1 involved in stable plasmid inheritance by postsegregational killing of, plasmid-less daughter cells. Kid forms a two-component system with its, antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a, 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory, toxin protein CcdB from E. coli F plasmid despite the lack of any notable, sequence similarity. Analysis of nontoxic mutants of Kid suggests a target, interaction interface associated with toxicity that is in marked contrast, to that proposed for CcdB. A possible region for interaction of Kid with, the antitoxin is proposed that overlaps with the target binding site and, may explain the mode of antitoxin action.

About this Structure

1M1F is a Single protein structure of sequence from Escherichia coli with PO4 as ligand. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of the kid toxin protein from E. coli plasmid R1., Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareno R, de la Cueva-Mendez G, Boelens R, Diaz-Orejas R, Rafferty JB, Structure. 2002 Oct;10(10):1425-33. PMID:12377128

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