2dpi

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[[Image:2dpi.gif|left|200px]]
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{{STRUCTURE_2dpi| PDB=2dpi | SCENE= }}
{{STRUCTURE_2dpi| PDB=2dpi | SCENE= }}
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'''Ternary complex of hPoli with DNA and dCTP'''
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===Ternary complex of hPoli with DNA and dCTP===
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==Overview==
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The 1,N6-ethenodeoxyadenosine (epsilon dA) lesion is promutagenic and has been implicated in carcinogenesis. We show here that human Pol iota, a Y-family DNA polymerase, can promote replication through this lesion by proficiently incorporating a nucleotide opposite it. The structural basis of this action is rotation of the epsilon dA adduct to the syn conformation in the Pol iota active site and presentation of its 'Hoogsteen edge' for hydrogen-bonding with incoming dTTP or dCTP. We also show that Pol zeta carries out the subsequent extension reaction and that efficiency of extension from epsilon dA x T is notably higher than from epsilon dA x C. Together, our studies reveal for the first time how the exocyclic epsilon dA adduct is accommodated in a DNA polymerase active site, and they show that the combined action of Pol iota and Pol zeta provides for efficient and error-free synthesis through this potentially carcinogenic DNA lesion.
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(as it appears on PubMed at http://www.pubmed.gov), where 16819516 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16819516}}
==About this Structure==
==About this Structure==
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[[Category: Ethenoda adduct]]
[[Category: Ethenoda adduct]]
[[Category: Lesion bypass]]
[[Category: Lesion bypass]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:54:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:30:52 2008''

Revision as of 05:30, 28 July 2008

Image:2dpi.png

Template:STRUCTURE 2dpi

Ternary complex of hPoli with DNA and dCTP

Template:ABSTRACT PUBMED 16819516

About this Structure

2DPI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Hoogsteen base pair formation promotes synthesis opposite the 1,N6-ethenodeoxyadenosine lesion by human DNA polymerase iota., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Nat Struct Mol Biol. 2006 Jul;13(7):619-25. Epub 2006 Jul 2. PMID:16819516

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