2eyi

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{{STRUCTURE_2eyi| PDB=2eyi | SCENE= }}
{{STRUCTURE_2eyi| PDB=2eyi | SCENE= }}
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'''Crystal structure of the actin-binding domain of human alpha-actinin 1 at 1.7 Angstrom resolution'''
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===Crystal structure of the actin-binding domain of human alpha-actinin 1 at 1.7 Angstrom resolution===
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==Overview==
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Alpha-actinin belongs to the spectrin family of actin crosslinking and bundling proteins that function as key regulators of cell motility, morphology and adhesion. The actin-binding domain (ABD) of these proteins consists of two consecutive calponin homology (CH) domains. Electron microscopy studies on ABDs appear to support two competing actin-binding models, extended and compact, whereas the crystal structures typically display a compact conformation. We have determined the 1.7A resolution structure of the ABD of alpha-actinin 1, a ubiquitously expressed isoform. The structure displays the classical compact conformation. We evaluated the two binding models by surface conservation analysis. The results show a conserved surface that spans both domains and corresponds to two previously identified actin-binding sites (ABS2 and ABS3). A third, and probably less important site, ABS1, is mostly buried in the compact conformation. However, a thorough examination of existing structures suggests a weak and semi-polar binding interface between the two CHs, leaving open the possibility of domain reorientation or opening. Our results are consistent with a two-step binding mechanism in which the ABD interacts first in the compact form observed in the structures, and then transitions toward a higher affinity state, possibly through minor rearrangement of the domains.
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(as it appears on PubMed at http://www.pubmed.gov), where 16698282 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16698282}}
==About this Structure==
==About this Structure==
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[[Category: Ch domain]]
[[Category: Ch domain]]
[[Category: Structural protein]]
[[Category: Structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:15:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:34:49 2008''

Revision as of 05:34, 28 July 2008

Image:2eyi.png

Template:STRUCTURE 2eyi

Crystal structure of the actin-binding domain of human alpha-actinin 1 at 1.7 Angstrom resolution

Template:ABSTRACT PUBMED 16698282

About this Structure

2EYI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the actin-binding domain of alpha-actinin 1: evaluating two competing actin-binding models., Borrego-Diaz E, Kerff F, Lee SH, Ferron F, Li Y, Dominguez R, J Struct Biol. 2006 Aug;155(2):230-8. Epub 2006 Apr 25. PMID:16698282

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