2fgq

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{{STRUCTURE_2fgq| PDB=2fgq | SCENE= }}
{{STRUCTURE_2fgq| PDB=2fgq | SCENE= }}
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'''High resolution X-ray structure of Omp32 in complex with malate'''
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===High resolution X-ray structure of Omp32 in complex with malate===
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==Overview==
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The porin Omp32 is the major outer membrane protein of the bacterium Delftia acidovorans. The crystal structures of the strongly anion-selective porin alone and in complex with the substrate malate were solved at 1.5 and 1.45 A resolution, respectively, and revealed a malate-binding motif adjacent to the channel constriction zone. Binding is mediated by interaction with a cluster of two arginine residues and two threonines. This binding site is specific for Omp32 and reflects the physiological adaptation of the organism to organic acids. Structural studies are combined with a 7-ns unbiased molecular dynamics simulation of the trimeric channel in a model membrane. Molecular dynamics trajectories show how malate ions are efficiently captured from the surrounding bulk solution by the electrostatic potential of the channel, translocated to the binding site region, and immobilized in the constriction zone. In accordance with these results, conductance measurements with Omp32 inserted in planar lipid membranes revealed binding of malate. The anion-selective channel Omp32 is the first reported example of a porin with a 16-stranded beta-barrel and proven substrate specificity. This finding suggests a new view on the correlation of porin structure with substrate binding in specific channels.
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{{ABSTRACT_PUBMED_16434398}}
==About this Structure==
==About this Structure==
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[[Category: Malate,outer membrane protein]]
[[Category: Malate,outer membrane protein]]
[[Category: Porin]]
[[Category: Porin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:52:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:38:37 2008''

Revision as of 05:38, 28 July 2008

Image:2fgq.png

Template:STRUCTURE 2fgq

High resolution X-ray structure of Omp32 in complex with malate

Template:ABSTRACT PUBMED 16434398

About this Structure

2FGQ is a Single protein structure of sequence from Delftia acidovorans. Full crystallographic information is available from OCA.

Reference

High resolution crystal structures and molecular dynamics studies reveal substrate binding in the porin Omp32., Zachariae U, Kluhspies T, De S, Engelhardt H, Zeth K, J Biol Chem. 2006 Mar 17;281(11):7413-20. Epub 2006 Jan 23. PMID:16434398

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