1m33

From Proteopedia

---

Revision as of 18:59, 20 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1m33, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of BioH at 1.7 A

Overview

Structural proteomics projects are generating three-dimensional structures, of novel, uncharacterized proteins at an increasing rate. However, structure alone is often insufficient to deduce the specific biochemical, function of a protein. Here we determined the function for a protein using, a strategy that integrates structural and bioinformatics data with, parallel experimental screening for enzymatic activity. BioH is involved, in biotin biosynthesis in Escherichia coli and had no previously known, biochemical function. The crystal structure of BioH was determined at 1.7, A resolution. An automated procedure was used to compare the structure of, BioH with structural templates from a variety of different enzyme active, sites. This screen identified a catalytic triad (Ser82, His235, and, Asp207) with a configuration similar to that of the catalytic triad of, hydrolases. Analysis of BioH with a panel of hydrolase assays revealed a, carboxylesterase activity with a preference for short acyl chain, substrates. The combined use of structural bioinformatics with, experimental screens for detecting enzyme activity could greatly enhance, the rate at which function is determined from structure.

About this Structure

1M33 is a Single protein structure of sequence from Escherichia coli with 3OH and EDO as ligands. Full crystallographic information is available from OCA.

Reference

Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from Escherichia coli., Sanishvili R, Yakunin AF, Laskowski RA, Skarina T, Evdokimova E, Doherty-Kirby A, Lajoie GA, Thornton JM, Arrowsmith CH, Savchenko A, Joachimiak A, Edwards AM, J Biol Chem. 2003 Jul 11;278(28):26039-45. Epub 2003 May 5. PMID:12732651

Page seeded by OCA on Tue Nov 20 21:06:27 2007